Placental Transfer of IgG and IgG Subclass Antibodies Anti‐Purified Escherichia coli LPS O16, O6 and O111

We evaluated 22 paired maternal and cord sera regarding the presence of IgG and IgG subclasses against purified Escherichia coli LPS O6, O16 and O111 employing ELISA for titre and avidity analysis, isoelectric focusing associated with affinity‐blotting for spectrotypic analysis, and the Western‐blot...

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Bibliographic Details
Published in:Scandinavian journal of immunology 1998-06, Vol.47 (6), p.609-614
Main Authors: Nagao, A T, Martinez, C C, Vieira, V S, Takano, O A, Costa-Carvalho, B T, Carneiro-Sampaio, M M
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antibodies, Bacterial - blood
Antibodies, Bacterial - classification
Antibodies, Bacterial - immunology
Antibody Affinity - immunology
Blotting, Western
Escherichia coli - immunology
Female
Humans
Immunity, Maternally-Acquired - immunology
Immunoblotting
Immunoglobulin G - blood
Immunoglobulin G - classification
Immunoglobulin G - immunology
Infant, Newborn
Isoelectric Focusing
Lipopolysaccharides - immunology
O Antigens - immunology
Placenta - immunology
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Summary:We evaluated 22 paired maternal and cord sera regarding the presence of IgG and IgG subclasses against purified Escherichia coli LPS O6, O16 and O111 employing ELISA for titre and avidity analysis, isoelectric focusing associated with affinity‐blotting for spectrotypic analysis, and the Western‐blotting technique for recognition of the various bands in lipopolysaccharide (LPS). Levels of anti‐LPS IgG antibodies in cord sera were equivalent to their respective maternal sera, showing a significant correlation (P 
ISSN:0300-9475
1365-3083
DOI:10.1046/j.1365-3083.1998.00353.x