An Antigen Recognized by Autologous CTLs on a Human Bladder Carcinoma

By stimulating blood lymphocytes with autologous bladder carcinoma cells that had been transfected with B7-1, we obtained a panel of CTL clones which lyse specifically the bladder tumor cells in an MHC class I-restricted fashion. Based on inhibition with anti-HLA Abs and the recognition of allogenei...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 1998-06, Vol.160 (12), p.6188-6194
Main Authors: Gueguen, Maryse, Patard, Jean-Jacques, Gaugler, Beatrice, Brasseur, Francis, Renauld, Jean-Christophe, Van Cangh, Paul J, Boon, Thierry, Van den Eynde, Benoit J
Format: Article
Language:English
Subjects:
Antigens, Neoplasm - genetics
Antigens, Neoplasm - immunology
Clone Cells - immunology
DNA, Complementary - chemistry
Humans
Point Mutation
Polymerase Chain Reaction
T-Lymphocytes, Cytotoxic - immunology
Tumor Cells, Cultured
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:By stimulating blood lymphocytes with autologous bladder carcinoma cells that had been transfected with B7-1, we obtained a panel of CTL clones which lyse specifically the bladder tumor cells in an MHC class I-restricted fashion. Based on inhibition with anti-HLA Abs and the recognition of allogeneic tumor cells, we could distribute our clones in three groups that recognized three distinct Ags. We characterized one of these Ags by screening a cDNA library prepared with the RNA from this bladder tumor line. This new tumor Ag is a peptide presented by HLA-B4403 molecules. It is produced by a point mutation in a gene that is recorded in databases under the name KIAA0205, is ubiquitously expressed, and whose function is unknown. We also found this mutation in the tumor sample that was originally resected from this patient, but the mutation was not found in the 100 or more independent tumors of various histologic types that were tested. This report is the first to describe the isolation of CTL clones directed against human bladder cancer and the molecular characterization of a bladder tumor Ag.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.160.12.6188