Salmonella abortusovis, strain Rv6, a new vaccinal vehicle for small ruminants

Salmonella abortusovis strain Rv6 ( Sao Rv6) is a live attenuated vaccine used for a few years to protect ewes against abortive salmonellosis. As Salmonellae, particularly Salmonella aro mutants, have considerable potential as vehicles for the presentation of heterologous vaccine antigens, Sao Rv6 w...

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Bibliographic Details
Published in:Veterinary microbiology 1998-03, Vol.61 (3), p.199-213
Main Authors: Bourgogne, A, Sanchis, R, Clément, J.M, Pépin, M
Format: Article
Language:English
Subjects:
Abortion, Veterinary - immunology
Abortion, Veterinary - prevention & control
Animals
Antibodies, Bacterial - biosynthesis
Antibodies, Bacterial - blood
Antibody Formation
ANTIGENE
ANTIGENOS
ANTIGENS
Bacterial Vaccines
Bacteriology
Biological and medical sciences
CAPRIN
CAPRINOS
Carrier Proteins - biosynthesis
Carrier Proteins - genetics
Carrier Proteins - immunology
Female
Fundamental and applied biological sciences. Psychology
GOATS
LIVE VACCINES
Male
Maltose-Binding Proteins
Mice
Microbiology
OVIN
OVINOS
PLASMIDE
PLASMIDIOS
PLASMIDS
Pregnancy
Ruminants
SALMONELLA
Salmonella - genetics
Salmonella - immunology
Salmonella abortusovis
Salmonella Infections, Animal - immunology
Salmonella Infections, Animal - prevention & control
SALMONELLOSE
SALMONELLOSIS
SALMONELOSIS
SHEEP
Sheep Diseases
VACCIN VIVANT
Vaccinal vehicle
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic
VACUNA VIVA
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Summary:Salmonella abortusovis strain Rv6 ( Sao Rv6) is a live attenuated vaccine used for a few years to protect ewes against abortive salmonellosis. As Salmonellae, particularly Salmonella aro mutants, have considerable potential as vehicles for the presentation of heterologous vaccine antigens, Sao Rv6 was tested in order to develop a vaccinal vehicle for small ruminants. Five vector plasmids were tested in Sao Rv6; these plasmids, which carry Maltose Binding Protein (MBP) expressed as protein, but differ in their promotors, had been previously tested in S. typhimurium strain SL3261, and were transferred into Sao Rv6. The five plasmids were stable in vitro, and the recombinant Sao Rv6 expressed MBP at various levels. Intraperitoneal infection of OF1 mice with the recombinant bacteria did not modify the characteristics of Sao Rv6: dissemination and infection levels were similar in all groups and all mice developed antibodies to Salmonella antigens as measured by ELISA. In contrast, only animals immunized with Sao Rv6 carrying the pNTE plasmid developed a serum antibody response to MBP. This plasmid was then tested in sheep; following subcutaneous immunization with Sao Rv6-pNTE, dissemination and infection levels were not modified in comparison with sheep immunized with Sao Rv6 lacking plasmid. Antibodies specific to MBP were detected in sera of sheep immunized with Sao Rv6-pNTE, purified MBP, and with S. typhimurium SL3261-pNTE as positive controls. These results demonstrate that Sao Rv6 can be used as a vehicle for heterologous antigens in sheep with pNTE as plasmid vector.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(98)00183-7