Differential Effects of Somatostatin and Its Analog on Protein Tyrosine Kinases Activity in the Rat Pituitary and the Murine Colonic Tumors

The effects of the native somatostatin-14 (SST-14) and of its analog octreotide (OCT) on the activity of protein tyrosine kinases (PTK) in the normal rat anterior pituitary gland, diethylstilbestrol (DES)-induced rat pituitary tumor and murine colonic cancer Colon 38 were studiedin vitro.PTK activit...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-05, Vol.246 (2), p.375-377
Main Authors: Pawlikowski, Marek, Lachowicz, Lilla, Kunert-Radek, Jolanta, Winczyk, Katarzyna, Janiszewska, Grażyna, Szkudlarek, Jolanta
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Hormonal - pharmacology
Colonic Neoplasms - drug therapy
Colonic Neoplasms - enzymology
Enzyme Inhibitors - pharmacology
In Vitro Techniques
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Octreotide - pharmacology
Pituitary Gland, Anterior - drug effects
Pituitary Gland, Anterior - enzymology
Pituitary Neoplasms - drug therapy
Pituitary Neoplasms - enzymology
Protein-Tyrosine Kinases - antagonists & inhibitors
Protein-Tyrosine Kinases - metabolism
Rats
Rats, Wistar
Receptors, Somatostatin - classification
Receptors, Somatostatin - drug effects
Receptors, Somatostatin - metabolism
Somatostatin - pharmacology
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Summary:The effects of the native somatostatin-14 (SST-14) and of its analog octreotide (OCT) on the activity of protein tyrosine kinases (PTK) in the normal rat anterior pituitary gland, diethylstilbestrol (DES)-induced rat pituitary tumor and murine colonic cancer Colon 38 were studiedin vitro.PTK activity was estimated in tissue homogenates using gamma-[32P]ATP and poly (Glu80, Tyr20) as a substrate. It was found that both SST-14 and OCT suppressed the PTK activity in all examined tissues. The suppressive effect was more pronounced in DES-induced pituitary tumor than in normal anterior pituitary gland, and in the former, OCT was more effective than SST-14. In contrast, SST-14 stronger suppressed PTK activity in colonic cancer than OCT. We hypothesize that SST-14 acts on PTK activity in colonic cancer mainly via SSTR-1 subtype of somatostatin receptors.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.8624