Solution Structure of the Glucocorticoid Receptor DNA-Binding Domain

The three-dimensional structure of the DNA-binding domain (DBD) of the glucocorticoid receptor has been determined by nuclear magnetic resonance spectroscopy and distance geometry. The structure of a 71-residue protein fragment containing two "zinc finger" domains is based on a large set o...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1990-07, Vol.249 (4965), p.157-160
Main Authors: Härd, Torleif, Kellenbach, Edwin, Boelens, Rolf, Maler, Bonnie A., Dahlman, Karin, Freedman, Leonard P., Carlstedt-Duke, Jan, Yamamoto, Keith R., Gustafsson, Jan-Åke, Kaptein, Robert
Format: Article
Language:English
Subjects:
Adrenocortical hormones
Amino Acid Sequence
Analysis
Animals
Atomic structure
Atoms
Biochemistry
Biological and medical sciences
Body regions
Cell receptors
Cell structures and functions
Corticosteroids
DNA
DNA - metabolism
DNA binding proteins
DNA-Binding Proteins - analysis
DNA-Binding Proteins - metabolism
Electronic structure
Fundamental and applied biological sciences. Psychology
Glucocorticoid receptors
Glucocorticoids
Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors
Humans
Magnetic Resonance Spectroscopy
Metalloproteins - analysis
Models, Molecular
Molecular and cellular biology
Molecular Sequence Data
Nuclear structure
Peptide Fragments - analysis
Peptide Fragments - metabolism
Protein Conformation
Rats
Receptors
Receptors, Glucocorticoid - analysis
Receptors, Glucocorticoid - metabolism
Regulatory Sequences, Nucleic Acid
Response elements
Zinc
Zinc - analysis
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Summary:The three-dimensional structure of the DNA-binding domain (DBD) of the glucocorticoid receptor has been determined by nuclear magnetic resonance spectroscopy and distance geometry. The structure of a 71-residue protein fragment containing two "zinc finger" domains is based on a large set of proton-proton distances derived from nuclear Overhauser enhancement spectra, hydrogen bonds in previously identified secondary structure elements, and coordination of two zinc atoms by conserved cysteine residues. The DBD is found to consist of a globular body from which the finger regions extend. A model of the dimeric complex between the DBD and the glucocorticoid response element is proposed. The model is consistent with previous results indicating that specific amino acid residues of the DBD are involved in protein-DNA and protein-protein interactions.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2115209