Immunohistochemical detection of ribosomal transcription factor UBF: diagnostic value in malignant specimens

The nucleolar organizer regions (NORs) of human chromosomes can be identified in interphase and mitotic cells by localization of some intrinsic components such as the associated enzyme RNA polymerase I. A new sensitive staining method for NORs is described using a specific antibody to the ribosomal...

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Bibliographic Details
Published in:The Journal of pathology 1998-01, Vol.184 (1), p.77-82
Main Authors: Torres-Montaner, Antonio, Bolivar, Jorge, Ortiz, Manuela, Valdivia, Manuel M.
Format: Article
Language:English
Subjects:
AgNORs
Biological and medical sciences
Biomarkers, Tumor - metabolism
DNA-Binding Proteins - immunology
DNA-Binding Proteins - metabolism
Fluorescent Antibody Technique
Host-tumor relations. Immunology. Biological markers
Humans
Immune Sera
Immunoenzyme Techniques
Lymphoma - diagnosis
Lymphoma - metabolism
Medical sciences
Neoplasm Proteins - metabolism
Neoplasms - diagnosis
Neoplasms - metabolism
nucleolar organizer regions
Nucleolus Organizer Region - metabolism
Pol1 Transcription Initiation Complex Proteins
Silver Staining
Transcription Factors - immunology
Transcription Factors - metabolism
Tumors
UBF
upstream binding factor
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Summary:The nucleolar organizer regions (NORs) of human chromosomes can be identified in interphase and mitotic cells by localization of some intrinsic components such as the associated enzyme RNA polymerase I. A new sensitive staining method for NORs is described using a specific antibody to the ribosomal transcription factor UBF. By indirect immunofluorescence and enzyme‐labelling methods, NORs stained in benign and malignant cells from a variety of tissues with monospecific anti‐UBF serum showed significant morphological differences which correlated well with histopathological evaluation. The number of NORs per cell in malignant preparations increased significantly. Furthermore, the staining of a NOR protein component such as UBF appears to be as sensitive as the silver‐staining technique (AgNOR) and might be a better alternative for detecting ribosomal activity in malignant tissues. © 1998 John Wiley & Sons, Ltd.
ISSN:0022-3417
1096-9896
DOI:10.1002/(SICI)1096-9896(199801)184:1<77::AID-PATH958>3.0.CO;2-X