Inhibition of sea urchin fertilization by jaspisin, a specific inhibitor of matrix metalloendoproteinase

Jaspisin, originally isolated from a marine sponge as an inhibitor of the hatching of the sea urchin (Hemicentrotus pulcherrimus) embryo, causes inhibition of sea urchin fertilization. Electron microscopic examination revealed that the acrosome reaction was induced in jaspisin‐treated sperm when the...

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Bibliographic Details
Published in:Development, growth & differentiation growth & differentiation, 1998-04, Vol.40 (2), p.221-230
Main Authors: Kato, Koichi H., Takemoto, Kazuhisa, Kato, Etsuko, Miyazaki, Kaoru, Kobayashi, Hiroki, Ikegami, Susumu
Format: Article
Language:English
Subjects:
Acrosome - drug effects
Acrosome - enzymology
Animals
Female
fertilization
Gelatinases - adverse effects
Hemicentrotus pulcherrimus
jaspisin
Male
Marine
Matrix Metalloproteinase 2
matrix metallo‐endoproteinase
Membrane Fusion - drug effects
Membrane Potentials - drug effects
Metalloendopeptidases - adverse effects
Metalloendopeptidases - antagonists & inhibitors
Protease Inhibitors - pharmacology
Sea Urchins - embryology
Sperm-Ovum Interactions - drug effects
Styrenes - pharmacology
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Summary:Jaspisin, originally isolated from a marine sponge as an inhibitor of the hatching of the sea urchin (Hemicentrotus pulcherrimus) embryo, causes inhibition of sea urchin fertilization. Electron microscopic examination revealed that the acrosome reaction was induced in jaspisin‐treated sperm when they were incubated with an intact egg. The acrosome‐reacted sperm bound to the vitelline layer by the acrosomal material surrounding the acrosomal process. However, fusion of the acrosomal process and the egg plasma membrane failed to take place. Membrane potential changes were monitored using eggs preloaded with a membrane potential‐sensitive fluorochrome, di‐8‐ANEPPS. Depolarization of the membrane potential, normally observed in the fertilized egg was not observed in the egg inseminated in the presence of jaspisin, indicating the absence of electrical continuity between the jaspisin‐treated egg and sperm. Jaspisin inhibited the activities of matrix metallo‐endoproteinase members but not of other types of proteinases. These results provide strong, albeit indirect, evidence that a matrix metallo‐endoproteinase(s) is involved in the process of gamete fusion during sea urchin fertilization.
ISSN:0012-1592
1440-169X
DOI:10.1046/j.1440-169X.1998.00011.x