Reduced ex vivo interleukin-8 production by neutrophils in septic and nonseptic systemic inflammatory response syndrome

Ex vivo cytokine production by circulating lymphocytes and monocytes is reduced in patients with infectious or noninfectious systemic inflammatory response syndrome. Very few studies have addressed the reactivity of polymorphonuclear cells (PMN). To analyze further the relative contribution of syste...

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Bibliographic Details
Published in:Blood 1998-05, Vol.91 (9), p.3439-3446
Main Authors: MARIE, C, MURET, J, FITTING, C, LOSSER, M.-R, PAYEN, D, CAVAILLON, J.-M
Format: Article
Language:English
Subjects:
Adult
Aged
Bacterial diseases
Bacterial sepsis
Biological and medical sciences
Endotoxins - pharmacology
Escherichia coli
Female
General Surgery
Human bacterial diseases
Humans
Infectious diseases
Interleukin-10 - physiology
Interleukin-8 - biosynthesis
Medical sciences
Middle Aged
Neutrophils - metabolism
Systemic Inflammatory Response Syndrome - physiopathology
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Summary:Ex vivo cytokine production by circulating lymphocytes and monocytes is reduced in patients with infectious or noninfectious systemic inflammatory response syndrome. Very few studies have addressed the reactivity of polymorphonuclear cells (PMN). To analyze further the relative contribution of systemic inflammatory response syndrome alone or in combination with infection we studied the interleukin-8 (IL-8) production by PMN isolated from patients who had undergone cardiac surgery with cardiopulmonary bypass (CPB) and patients with sepsis. Cells were activated with either lipopolysaccharide (LPS) or heat-killed streptococci. Compared with healthy controls, the release of IL-8 by PMN in both groups of patients was significantly reduced whether activated by LPS, independently of its concentration and origin, or by heat-killed streptococci. These observations suggest that stressful conditions related to inflammation, independently of infection, rapidly dampened the reactivity of circulating PMN. We investigated whether the observed diminished reactivity of PMN might reflect an endotoxin tolerance phenomenon. Our in vitro experiments with PMN from healthy controls indicated that PMN could not be rendered tolerant stricto sensu. However, our data suggested that LPS-induced mediators such as IL-10 may be responsible for the observed anergy in patients.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V91.9.3439