Fibronectin Production by Cultured Human Lung Fibroblasts in Three-Dimensional Collagen Gel Culture

In vivo, fibroblasts are distributed in a three-dimensional (3-D) connective tissue matrix. Fibronectin is a major product of fibroblasts in routine cell culture and is thought to regulate many aspects of fibroblast biology. In this context, we sought to determine if the interaction of fibroblasts w...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 1998-03, Vol.34 (3), p.203-210
Main Authors: Yuichi Adachi, Tadashi Mio, Keiichi Takigawa, Ilja Striz, Debra J. Romberger, John R. Spurzem, Rennard, Stephen I.
Format: Article
Language:English
Subjects:
Blotting, Northern
Cell Count
Cell Culture Techniques
Cellular Models
Collagen
Collagens
Culture media
Cultured cells
DNA
Epithelial cells
Error rates
Fiber cells
Fibroblasts
Fibroblasts - metabolism
Fibronectins - biosynthesis
Gels
Humans
Lung - metabolism
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Summary:In vivo, fibroblasts are distributed in a three-dimensional (3-D) connective tissue matrix. Fibronectin is a major product of fibroblasts in routine cell culture and is thought to regulate many aspects of fibroblast biology. In this context, we sought to determine if the interaction of fibroblasts with a 3-D matrix might affect fibronectin production. To examine this hypothesis, fibronectin production by fibroblasts cultured in a 3-D collagen gel or on plastic dishes was measured by ELISA. Fibroblasts in 3-D gel culture produced more fibronectin than those in monolayer culture. Fibroblasts in 3-D culture produced increasing amounts of fibronectin when the collagen concentration of the gel was increased. The 3-D nature of the matrix appeared to be crucial because plating the fibroblasts on the surface of a plastic dish underneath a collagen gel was not different from plating them on a plastic dish in the absence of collagen. In addition to increased fibronectin production, the distribution of the fibronectin produced in 3-D culture was different from that of monolayer culture. In monolayer culture, more than half of the fibronectin was released into the culture medium. In 3-D culture, however, approximately two-thirds remained in the collagen gel. In summary, the presence of a 3-D collagen matrix increases fibroblast fibronectin production and results in greater retention of fibronectin in the vicinity of the producing cells.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-998-0125-7