In vitro engineering of human skin-like tissue

Coverage of large, full‐thickness burns presents a challenge for the surgeon due to the lack of availability of the patient's own skin. Currently, tissue engineering offers the possibility of performing a suitable therapeutic wound coverage after early burn excision by using cultured keratinocy...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research 1998-05, Vol.40 (2), p.187-194
Main Authors: Zacchi, Valentina, Soranzo, Carlo, Cortivo, Roberta, Radice, Marco, Brun, Paola, Abatangelo, Giovanni
Format: Article
Language:English
Subjects:
Biocompatible Materials
Biological and medical sciences
Burns - therapy
Cell Division
Coculture Techniques
extracellular matrix
Extracellular Matrix Proteins - metabolism
Fibroblasts - cytology
Fibroblasts - metabolism
Humans
hyaluronic acid derivatives
Immunohistochemistry
integrins
Keratinocytes - cytology
Keratinocytes - metabolism
Medical sciences
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
skin substitute
Skin, Artificial
Technology. Biomaterials. Equipments. Material. Instrumentation
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Coverage of large, full‐thickness burns presents a challenge for the surgeon due to the lack of availability of the patient's own skin. Currently, tissue engineering offers the possibility of performing a suitable therapeutic wound coverage after early burn excision by using cultured keratinocyte sheets supported by a dermal layer. The aim of this study was to develop and characterize a skin substitute composed of both epidermal and dermal elements. For this purpose we grew keratinocytes and fibroblasts separately for 15 days within two different types of biomaterials. Cells then were co‐cultured for an additional period of 15 days, after which samples were taken and processed with either classic or immunohistochemical stainings. Results showed that (1) human fibroblasts and keratinocytes can be cultured on hyaluronic acid‐derived biomaterials and that (2) the pattern of expression of particular dermal–epidermal molecules is similar to that found in normal skin. The data from this study suggest that our skin equivalent might be useful in the treatment of both burns and chronic wounds. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 40, 187–194, 1998.
ISSN:0021-9304
1097-4636
DOI:10.1002/(SICI)1097-4636(199805)40:2<187::AID-JBM3>3.0.CO;2-H