Juvenile hormone acid: evidence for a hormonal function in induction of vitellogenin in larvae of Manduca sexta

In the tobacco hornworm (Manduca sexta), vitellogenin (Vg), the major yolk protein precursor, and its mRNA are first detectable in the prepupal stage; and production of both can be enhanced by methoprene, a juvenile hormone (JH) analog. Competence to respond to methoprene is acquired after ecdystero...

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Bibliographic Details
Published in:Archives of insect biochemistry and physiology 1998, Vol.37 (4), p.305-314
Main Authors: Ismail, S.M. (Texas AandM University, College Station, TX.), Satyanarayana, K, Bradfield, J.Y, Dahm, K.H, Bhaskaran, G
Format: Article
Language:English
Subjects:
Abdomen
ANALOGS
Animals
CONTROL HORMONAL
CONTROLE HORMONAL
ECDYSTERONE
ESTEROIDES
Gene Expression Regulation, Developmental - drug effects
Hemolymph - physiology
HORMONAL CONTROL
hormonal induction
HORMONAS DE LA MUDA
HORMONAS JUVENILES
HORMONE DE MUE
HORMONE JUVENILE
Hydrazines - pharmacology
Insect Hormones - pharmacology
juvenile hormone acid
JUVENILE HORMONE II-ACID
JUVENILE HORMONES
Juvenile Hormones - pharmacology
Larva
LARVAE
LARVAS
LARVE
Manduca - physiology
MANDUCA SEXTA
Metamorphosis, Biological
METHOPRENE
Methoprene - pharmacology
methoprene acid
METOPRENO
MOULTING HORMONES
OOGENESIS
OVOGENESE
RH-5992
RNA, Messenger - biosynthesis
STEROIDE
STEROIDS
Transcription, Genetic - drug effects
VITELLOGENESIS
vitellogenin
VITELLOGENINE
VITELLOGENINS
Vitellogenins - biosynthesis
Vitellogenins - isolation & purification
VITELOGENINA
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Summary:In the tobacco hornworm (Manduca sexta), vitellogenin (Vg), the major yolk protein precursor, and its mRNA are first detectable in the prepupal stage; and production of both can be enhanced by methoprene, a juvenile hormone (JH) analog. Competence to respond to methoprene is acquired after ecdysteroid-initiated commitment for metamorphosis. Here we show that acquisition of competence requires prior exposure to JH-II acid in addition to ecdysteroid. Application of 20-hydroxyecdysone or RH5992, an ecdysteroid analog, to isolated abdomens from feeding larvae (precommitment) results in exposure of the dorsal vessel (EDV), a sign of metamorphic commitment-but such abdomens do not make Vg in response to methoprene. However, injection of JH-II acid along with 20-hydroxyecdysone into isolated abdomens causes Vg production in response to methoprene. Methoprene acid similarly induces competence to respond to methoprene. Northern blot analysis confirmed that Vg transcripts are present in fat body only if isolated abdomens were pretreated with both ecdysteroid, and JH-II acid or methoprene acid. The latter two can induce competence even in precocious prepupae resulting from removal of the corpora allata (the glands that produce JH) from early penultimate larvae. JH-III acid and related metabolites such as farnesol, farnesoic acid, and methyl farnesoate do not induce competence. Hitherto, JH acids have been regarded as precursors or catabolites of JHs. Here we show for the first time that JH acid has a hormonal function that cannot be performed by JH itself
ISSN:0739-4462
1520-6327
DOI:10.1002/(SICI)1520-6327(1998)37:4<305::AID-ARCH6>3.0.CO;2-P