Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Abstract The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404±40 kb. The chromosome sizes for the reference strains of the other...

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Bibliographic Details
Published in:FEMS microbiology letters 1998-03, Vol.160 (2), p.209-216
Main Authors: Chevallier, Bruno, Dugourd, Dominique, Tarasiuk, Kazimirez, Harel, Josée, Gottschalk, Marcelo, Kobisch, Marylène, Frey, Joachim
Format: Article
Language:English
Subjects:
Actinobacillus pleuropneumoniae
Actinobacillus pleuropneumoniae - classification
Actinobacillus pleuropneumoniae - genetics
Animals
Asci
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Chromosome size
Chromosomes
Deoxyribonucleic acid
DNA
DNA Restriction Enzymes
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Food processing industry
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Genetics
Genome, Bacterial
Genomes
Heterogeneity
Microbiology
Phylogenetics
Phylogeny
Polymorphism
Polymorphism, Restriction Fragment Length
Pulsed field gel electrophoresis
Serotypes
Serotyping
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Summary:Abstract The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404±40 kb. The chromosome sizes for the reference strains of the other serotypes range between 2.3 and 2.4 Mb. The restriction pattern profiles of AscI, ApaI and NheI digested chromosomes showed a high degree of polymorphism among the different serotype reference strains and allowed their discrimination. The analysis of the macrorestriction pattern polymorphism revealed phylogenetic relationships between the different serotype reference strains which reflect to some extent groups of serotypes known to cross-react serologically. In addition, different pulsed fields gel electrophoresis patterns also revealed heterogeneity in the chromosomal structure among different field strains of serotypes 1, 5a, and 5b, while strains of serotype 9 originating from most distant geographical places showed homogeneous ApaI patterns in pulsed field gel electrophoresis.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1998.tb12913.x