Reduced E-cadherin immunohistochemical expression in node-negative breast carcinomas correlates with 10-year survival

E-cadherin immunodetection was performed on frozen sections, using an immunoperoxidase procedure and with computer-assisted analysis of digitized colored microscopic images in a series of 179 breast carcinomas. Quantitative immunocytochemical assays were correlated with follow-up (129 months). The r...

Full description

Saved in:
Bibliographic Details
Published in:American journal of clinical pathology 1998-04, Vol.109 (4), p.431-438
Main Authors: CHARPIN, C, GARCIA, S, BONNIER, P, MARTINI, F, ANDRAC, L, CHOUX, R, LAVAUT, M.-N, ALLASIA, C
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Breast Neoplasms - metabolism
Breast Neoplasms - mortality
Cadherins - metabolism
Carcinoma - metabolism
Carcinoma - mortality
Disease-Free Survival
Female
Follow-Up Studies
Gynecology. Andrology. Obstetrics
Humans
Image Processing, Computer-Assisted
Immunohistochemistry
Lymph Nodes - pathology
Mammary gland diseases
Medical sciences
Middle Aged
Multivariate Analysis
Prognosis
Retrospective Studies
Survival Rate
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:E-cadherin immunodetection was performed on frozen sections, using an immunoperoxidase procedure and with computer-assisted analysis of digitized colored microscopic images in a series of 179 breast carcinomas. Quantitative immunocytochemical assays were correlated with follow-up (129 months). The results showed that reduced E-cadherin immunocytochemical expression in tumors (cut point, 4%) significantly correlated with shorter overall survival in node-negative patients (Kaplan Meier log rank test). But E-cadherin immunostained expression (cut point, 4%) did not correlate with metastasis-free or recurrence-free survival. In multivariate analysis (Cox proportional hazards regression model), E-cadherin prognostic significance for overall survival in node-negative patients was independent of the tumor size, grade, and histologic type. The results suggest that reduced E-cadherin expression detected in optimum technical conditions (frozen samples and quantitative immunohistochemistry) is an independent indicator of poor survival in node-negative patients and may be clinically relevant for the treatment of patients with breast carcinomas.
ISSN:0002-9173
1943-7722
DOI:10.1093/ajcp/109.4.431