Use of liquid chromatography–tandem mass spectrometry for the quantitative and qualitative analysis of an antipsychotic agent and its metabolites in human plasma and urine

The use of liquid chromatography–tandem mass spectrometry (LC–MS–MS) for the quantitative analysis of an `atypical' antipsychotic agent in human plasma is described. The method uses atmospheric pressure chemical ionisation, and offers increased sensitivity, selectivity and speed of analysis ove...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-01, Vol.794 (1-2), p.27-36
Main Authors: Dear, Gordon J, Fraser, Ian J, Patel, Dipak K, Long, Jeffrey, Pleasance, Stephen
Format: Article
Language:English
Subjects:
2-amino-N-(4-(4-(1,2-benzisothiazol-3-yl)-1-piperazinyl)butyl) benzamide
Antipsychotic agent
Antipsychotic Agents - analysis
Antipsychotic Agents - blood
Antipsychotic Agents - urine
Calibration
Chromatography, Liquid
Double-Blind Method
Humans
Mass Spectrometry
Spectrometry, Fluorescence
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Summary:The use of liquid chromatography–tandem mass spectrometry (LC–MS–MS) for the quantitative analysis of an `atypical' antipsychotic agent in human plasma is described. The method uses atmospheric pressure chemical ionisation, and offers increased sensitivity, selectivity and speed of analysis over an existing high-performance liquid chromatography method using fluorescence detection. This method enabled same day turn around of results for in excess of 100 samples, including sample preparation, data acquisition and processing. LC–MS–MS was also used to detect and characterise known and unknown in vivo metabolites of the drug in human urine and plasma, using electrospray ionisation.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00688-2