Cryptosporidium parvum development in the BS-C-1 cell line

Cryptosporidium parvum is a worldwide parasitic protozoon capable of causing life-threatening disease in immunocompromised patients. In vitro cultivation of C. parvum has been under investigation for development of a well-defined in vitro model for C. parvum infectivity assay. This is the first repo...

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Bibliographic Details
Published in:The Journal of parasitology 1998-02, Vol.84 (1), p.8-15
Main Authors: Deng, M.Q, Cliver, D.O
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
biological development
Cell culture techniques
Cell Line
cell lines
Cercopithecus aethiops
Cryptosporidium parvum
Cryptosporidium parvum - genetics
Cryptosporidium parvum - growth & development
Cryptosporidium parvum - immunology
Development
DNA
DNA, Protozoan - analysis
fluorescent antibody technique
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Immune Sera - immunology
in vitro culture
Infections
life cycle (organisms)
Liquids
Microbiology
Oocysts
pathogenicity
Polymerase Chain Reaction
Protozoa
Sodium
Species Specificity
Sporozoites
Viability
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Summary:Cryptosporidium parvum is a worldwide parasitic protozoon capable of causing life-threatening disease in immunocompromised patients. In vitro cultivation of C. parvum has been under investigation for development of a well-defined in vitro model for C. parvum infectivity assay. This is the first report of C. parvum completing its life cycle in BS-C-1, an African green monkey kidney cell line. Both sodium hypochlorite-stimulated oocysts and purified sporozoites were able to initiate infection that led to completion of the whole life cycle, although inoculating purified sporozoites was less efficient. Beside Giemsa staining and normal light microscopy, an indirect fluorescent antibody staining method was developed to facilitate the detection and interpretation of C. parvum life stages developed in vitro. A C. parvum-specific polymerase chain reaction was also applied to detect and confirm the presence of C. parvum life stages in liquid from oocyst-infected cell cultures. In addition to the 452-base-pair (bp) product that can be specifically amplified from C. parvum oocysts and sporozoites, a fragment near 280 bp was also obtained. Various applications of this in vitro culture system are envisioned.
ISSN:0022-3395
1937-2345
DOI:10.2307/3284519