Effects of inaccurate reference lifetimes on interpreting frequency-domain fluorescence data

The effects of assigning inaccurate reference lifetimes in lifetime determinations are predicted theoretically by using standard equations. This theory leads to a method to remove reference error effects using common least-squares software. This method cannot, however, be used to deconvolute data co...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1990-03, Vol.62 (5), p.471-476
Main Authors: Litwiler, Kevin S, Huang, Jingfan, Bright, Frank V
Format: Article
Language:English
Subjects:
Atomic and molecular physics
Exact sciences and technology
Intensities and shapes of molecular spectral lines and bands
Kinetics
Molecular properties and interactions with photons
Physics
Reference Standards
Spectrometry, Fluorescence - standards
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Description
Summary:The effects of assigning inaccurate reference lifetimes in lifetime determinations are predicted theoretically by using standard equations. This theory leads to a method to remove reference error effects using common least-squares software. This method cannot, however, be used to deconvolute data collected with isochronal references. Uncorrected data can always be exactly solved with models containing one more degree of freedom than the true model. Monoexponential decays are fit by double-exponential decays or excited-state processes. Unimodal distributed decays often appear as discrete, double-exponential decays.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00204a010