Construction of gene libraries for each human chromosome

We describe the construction of two complete sets of small insert, complete digest DNA libraries for each of the 24 human chromosomal types by the National Laboratory Gene Library Project. Flow sorting was used to purify the chromosomes which provided the DNA for cloning. One set of libraries was cl...

Full description

Saved in:
Bibliographic Details
Published in:Cytometry (New York, N.Y.) N.Y.), 1990, Vol.11 (1), p.208-218
Main Authors: Van Dilla, Marvin A., Deaven, Larry L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Fractionation
Charon 21A
Chromatin. Chromosome
Chromosome Mapping
chromosomes
Chromosomes - analysis
Chromosome‐specific
Cloning, Molecular
cosmid vectors
DNA cloning
DNA libraries
Flow Cytometry
flow sorting
Fundamental and applied biological sciences. Psychology
Gene Library
genes
Genome, Human
Humans
lambda vectors
Molecular and cellular biology
Molecular genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We describe the construction of two complete sets of small insert, complete digest DNA libraries for each of the 24 human chromosomal types by the National Laboratory Gene Library Project. Flow sorting was used to purify the chromosomes which provided the DNA for cloning. One set of libraries was cloned into the HindIII site of the lambda vector Charon 21A, and the other set was cloned into the EcoRI site of the same vector. Characterization information from both in‐house experiments and user feedback is presented. These chromosome‐specific libraries are available to the general scientific community from a repository at the American Type Culture Collection, Rockville, MD. The second phase of the project, the construction of large insert, partial digest libraries in both lambda and cosmid vectors, is underway.
ISSN:0196-4763
1097-0320
DOI:10.1002/cyto.990110124