Sensitive and reliable detection of genomic imbalances in human neuroblastomas using comparative genomic hybridisation analysis

Deletions of the short arm of chromosome 1, extra copies of chromosome 17q and MYCN amplification are the most frequently encountered genetic changes in neuroblastomas. Standard techniques for detection of one or more of these genetic changes are karyotyping, FISH analysis and LOH analysis by Southe...

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Bibliographic Details
Published in:European journal of cancer (1990) 1997-10, Vol.33 (12), p.1979-1982
Main Authors: Van Gele, M, Van Roy, N, Jauch, A, Laureys, G, Benoit, Y, Schelfhout, V, De Potter, C.R, Brock, P, Uyttebroeck, A, Sciot, R, Schuuring, E, Versteeg, R, Speleman, F
Format: Article
Language:English
Subjects:
Child, Preschool
childhood
Chromosome Deletion
Chromosomes, Human, Pair 1 - genetics
Chromosomes, Human, Pair 17 - genetics
comparative genomic hybridisation
Gene Amplification - genetics
Genes, myc - genetics
genetic imbalances
genetics
Humans
Male
neuroblastoma
Neuroblastoma - genetics
Nucleic Acid Hybridization - methods
Sensitivity and Specificity
Tumor Cells, Cultured
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Summary:Deletions of the short arm of chromosome 1, extra copies of chromosome 17q and MYCN amplification are the most frequently encountered genetic changes in neuroblastomas. Standard techniques for detection of one or more of these genetic changes are karyotyping, FISH analysis and LOH analysis by Southern blot or PCR. Each of these techniques has its own particular limitations. More recently, comparative genomic hybridisation (CGH) was introduced for detection of genomic imbalances including deletions, duplications and gene amplification. We evaluated the sensitivity and reliability of CGH for detection of the most frequently encountered genetic changes in neuroblastoma. For this purpose a panel of well-characterised neuroblastoma cell lines as well as a series of 11 primary neuroblastomas was analysed. Our results show that CGH is a valuable tool for the genetic characterisation of neuroblastomas, both for the detection of frequently occurring genomic imbalances and for the identification of previously unnoticed genetic changes.
ISSN:0959-8049
1879-0852
DOI:10.1016/S0959-8049(97)00289-X