Enzyme-linked immunosorbent assay using F(ab′) 2 fragment for the detection of human pulmonary surfactant protein D in sera

We developed an enzyme-linked immunosorbent assay (ELISA) for detection of SP-D in serum using recombinant SP-D as a standard and horseradish peroxidase conjugated F(ab′) 2 fragment of mouse monoclonal antibody IgG to avoid the interaction of serum factors including rheumatoid factor. The use of F(a...

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Bibliographic Details
Published in:Clinica chimica acta 1997-10, Vol.266 (2), p.157-171
Main Authors: Nagae, Hisato, Takahashi, Hiroki, Kuroki, Yoshio, Honda, Yasuhito, Nagata, Atsuo, Ogasawara, Yoshinori, Abe, Shosaku, Akino, Toyoaki
Format: Article
Language:English
Subjects:
Adult
Animals
Antibodies, Monoclonal
Collagen disease
Collagen Diseases - blood
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Glycoproteins - blood
Horseradish Peroxidase
Humans
Immunoglobulin Fragments
Immunoglobulin G
Interstitial pneumonia
Lung Diseases, Interstitial - blood
Mice
Middle Aged
Pulmonary Alveolar Proteinosis - blood
Pulmonary Fibrosis - blood
Pulmonary surfactant protein D
Pulmonary Surfactant-Associated Protein D
Pulmonary Surfactants - blood
Serum
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Summary:We developed an enzyme-linked immunosorbent assay (ELISA) for detection of SP-D in serum using recombinant SP-D as a standard and horseradish peroxidase conjugated F(ab′) 2 fragment of mouse monoclonal antibody IgG to avoid the interaction of serum factors including rheumatoid factor. The use of F(ab′) 2 fragment dramatically decreased the value of serum SP-D concentration in rheumatoid arthritis patients without pulmonary complication to the close level of healthy volunteer. In contrast, the patients with collagen disease having interstitial pulmonary pneumonia exhibited consistently elevated levels of serum SP-D. The use of new ELISA with recombinant SP-D and F(ab′) 2 fragment of anti-SP-D monoclonal antibody gives a greater advantage for the accurate detection of SP-D in sera from patients with idiopathic pulmonary fibrosis, interstitial pneumonia with collagen disease and pulmonary alveolar proteinosis without interference of rheumatoid factor.
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(97)00124-1