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Expression of cereolysine AB genes in Bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection
The cereolysin AB genes from Bacillus cereus VKM-B164 have been expressed in Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated wi...
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| Published in: | Vaccine 1997-12, Vol.15 (17), p.1846-1850 |
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| Main Authors: | , , , |
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| Language: | eng ; rus |
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| container_end_page | 1850 |
| container_issue | 17 |
| container_start_page | 1846 |
| container_title | Vaccine |
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| creator | Pomerantsev, A.P. Staritsin, N.A. Mockov, Yu.V. Marinin, L.I. |
| description | The cereolysin AB genes from
Bacillus cereus VKM-B164 have been expressed in
Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were vaccinated with parental and recombinant
B. anthracis STI-1 and 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains showed absence of protection. STI-1 immunisation protected against the H-7 strain, but did not protect against the recombinant strain. STI-1 recombinant strain protected gold hamsters against the H-7 as well as the recombinant H-7 strains. The results describe the modulation of immunopathogenic properties of
B. anthracis due to expression of cereolysin AB genes. |
| doi_str_mv | 10.1016/S0264-410X(97)00132-1 |
| format | article |
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Bacillus cereus VKM-B164 have been expressed in
Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were vaccinated with parental and recombinant
B. anthracis STI-1 and 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains showed absence of protection. STI-1 immunisation protected against the H-7 strain, but did not protect against the recombinant strain. STI-1 recombinant strain protected gold hamsters against the H-7 as well as the recombinant H-7 strains. The results describe the modulation of immunopathogenic properties of
B. anthracis due to expression of cereolysin AB genes.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(97)00132-1</identifier><identifier>PMID: 9413092</identifier><identifier>CODEN: VACCDE</identifier><language>eng ; rus</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Anthrax - prevention & control ; Bacillus anthracis ; Bacillus anthracis - genetics ; Bacillus anthracis - immunology ; Bacillus anthracis - metabolism ; Bacillus cereus ; Bacillus cereus - genetics ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Bacterial Vaccines - genetics ; Bacterial Vaccines - immunology ; Bacterial Vaccines - therapeutic use ; Cricetinae ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; Electroporation ; Genes, Bacterial ; Hemolysin Proteins - biosynthesis ; Mesocricetus ; Mice ; modulation of immunopathogenicity ; Nucleic Acid Hybridization ; Plasmids - genetics ; Transformation, Bacterial</subject><ispartof>Vaccine, 1997-12, Vol.15 (17), p.1846-1850</ispartof><rights>1997</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-dee20cc811dbc5e0c06dd4368f7ae05dbd73b7d9d1c147b86973b8c4c1ce3ace3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2071287$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9413092$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pomerantsev, A.P.</creatorcontrib><creatorcontrib>Staritsin, N.A.</creatorcontrib><creatorcontrib>Mockov, Yu.V.</creatorcontrib><creatorcontrib>Marinin, L.I.</creatorcontrib><title>Expression of cereolysine AB genes in Bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>The cereolysin AB genes from
Bacillus cereus VKM-B164 have been expressed in
Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were vaccinated with parental and recombinant
B. anthracis STI-1 and 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains showed absence of protection. STI-1 immunisation protected against the H-7 strain, but did not protect against the recombinant strain. STI-1 recombinant strain protected gold hamsters against the H-7 as well as the recombinant H-7 strains. The results describe the modulation of immunopathogenic properties of
B. anthracis due to expression of cereolysin AB genes.</description><subject>Animals</subject><subject>Anthrax - prevention & control</subject><subject>Bacillus anthracis</subject><subject>Bacillus anthracis - genetics</subject><subject>Bacillus anthracis - immunology</subject><subject>Bacillus anthracis - metabolism</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus - genetics</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Vaccines - genetics</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacterial Vaccines - therapeutic use</subject><subject>Cricetinae</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Electroporation</subject><subject>Genes, Bacterial</subject><subject>Hemolysin Proteins - biosynthesis</subject><subject>Mesocricetus</subject><subject>Mice</subject><subject>modulation of immunopathogenicity</subject><subject>Nucleic Acid Hybridization</subject><subject>Plasmids - genetics</subject><subject>Transformation, Bacterial</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxi0EKsvCI1TyAaFyCHjsJE5OqK1Ki1SJAyBxs5zxpDXKOoudVNtn4KXxdqO99mBZo_nNN38-xk5BfAIB9ecfQtZlUYL4fdbqj0KAkgW8YCtotCpkBc1Ltjoir9mblP4IISoF7Qk7aUtQopUr9u9qt42Ukh8DH3uOFGkcHpMPxM8v-B0FStwHfmHRD8OcuA3TfcxB4g8WcY-lKdpMUEhzFuLbOE6E017P3uVEmjjtthT9hsJkB35Pm9xg8rhI7bJ8fyh4y171dkj0bvnX7NfXq5-XN8Xt9-tvl-e3BZZaToUjkgKxAXAdViRQ1M6Vqm56bUlUrnNaddq1DhBK3TV1m-MGSwQkZfNbsw8H3Tzr35nSZDY-IQ2DDTTOyei2bJXQ8lkQagWlVHUGqwOIcUwpUm-2eWEbHw0Is3fLPLll9laYVpsntwzkutOlwdxtyB2rFnty_v2Stwnt0Ecb8umPmBQaZLZ7zb4cMMpXe_AUTUJPAcn5mE9r3OifGeQ_Q_O11g</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Pomerantsev, A.P.</creator><creator>Staritsin, N.A.</creator><creator>Mockov, Yu.V.</creator><creator>Marinin, L.I.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>Expression of cereolysine AB genes in Bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection</title><author>Pomerantsev, A.P. ; 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Bacillus cereus VKM-B164 have been expressed in
Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were vaccinated with parental and recombinant
B. anthracis STI-1 and 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains showed absence of protection. STI-1 immunisation protected against the H-7 strain, but did not protect against the recombinant strain. STI-1 recombinant strain protected gold hamsters against the H-7 as well as the recombinant H-7 strains. The results describe the modulation of immunopathogenic properties of
B. anthracis due to expression of cereolysin AB genes.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9413092</pmid><doi>10.1016/S0264-410X(97)00132-1</doi><tpages>5</tpages></addata></record> |
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| ispartof | Vaccine, 1997-12, Vol.15 (17), p.1846-1850 |
| issn | 0264-410X 1873-2518 |
| language | eng ; rus |
| recordid | cdi_proquest_miscellaneous_79493072 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Anthrax - prevention & control Bacillus anthracis Bacillus anthracis - genetics Bacillus anthracis - immunology Bacillus anthracis - metabolism Bacillus cereus Bacillus cereus - genetics Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Bacterial Vaccines - genetics Bacterial Vaccines - immunology Bacterial Vaccines - therapeutic use Cricetinae DNA, Bacterial - analysis DNA, Bacterial - genetics Electroporation Genes, Bacterial Hemolysin Proteins - biosynthesis Mesocricetus Mice modulation of immunopathogenicity Nucleic Acid Hybridization Plasmids - genetics Transformation, Bacterial |
| title | Expression of cereolysine AB genes in Bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection |
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