Expression of cereolysine AB genes in Bacillus anthracis vaccine strain ensures protection against experimental hemolytic anthrax infection

The cereolysin AB genes from Bacillus cereus VKM-B164 have been expressed in Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated wi...

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Bibliographic Details
Published in:Vaccine 1997-12, Vol.15 (17), p.1846-1850
Main Authors: Pomerantsev, A.P., Staritsin, N.A., Mockov, Yu.V., Marinin, L.I.
Format: Article
Language:eng ; rus
Subjects:
Animals
Anthrax - prevention & control
Bacillus anthracis
Bacillus anthracis - genetics
Bacillus anthracis - immunology
Bacillus anthracis - metabolism
Bacillus cereus
Bacillus cereus - genetics
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Bacterial Vaccines - genetics
Bacterial Vaccines - immunology
Bacterial Vaccines - therapeutic use
Cricetinae
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Electroporation
Genes, Bacterial
Hemolysin Proteins - biosynthesis
Mesocricetus
Mice
modulation of immunopathogenicity
Nucleic Acid Hybridization
Plasmids - genetics
Transformation, Bacterial
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Summary:The cereolysin AB genes from Bacillus cereus VKM-B164 have been expressed in Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were vaccinated with parental and recombinant B. anthracis STI-1 and 221 strains and challenged with virulent ones subcutaneously. Gold hamsters vaccinated with 221 strains showed absence of protection. STI-1 immunisation protected against the H-7 strain, but did not protect against the recombinant strain. STI-1 recombinant strain protected gold hamsters against the H-7 as well as the recombinant H-7 strains. The results describe the modulation of immunopathogenic properties of B. anthracis due to expression of cereolysin AB genes.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(97)00132-1