Identification of a novel nuclear speckle-type protein, SPOP

A novel antigen recognized by serum from a scleroderma patient was identified by expression cloning from the HeLa cell cDNA library. The cloned cDNA encoded a 374-amino acid protein with a relative molecular mass of 47 000 and a predicted amino acid sequence 62.7% identical to the hypothetical prote...

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Bibliographic Details
Published in:FEBS letters 1997-11, Vol.418 (1), p.23-26
Main Authors: Nagai, Yasuo, Kojima, Tatsuya, Muro, Yoshinao, Hachiya, Takahisa, Nishizawa, Yuji, Wakabayashi, Takashi, Hagiwara, Masatoshi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Autoantibody
Caenorhabditis elegans
cDNA cloning
Cloning, Molecular
Conserved Sequence
COS Cells
DNA, Complementary
Evolution, Molecular
Gene Library
HeLa Cells
Humans
Molecular Sequence Data
Nuclear protein
Nuclear Proteins - biosynthesis
Nuclear Proteins - chemistry
POZ domain
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Repressor Proteins
RNA, Messenger - biosynthesis
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Tagged Sites
Transcription, Genetic
Transfection
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Summary:A novel antigen recognized by serum from a scleroderma patient was identified by expression cloning from the HeLa cell cDNA library. The cloned cDNA encoded a 374-amino acid protein with a relative molecular mass of 47 000 and a predicted amino acid sequence 62.7% identical to the hypothetical protein of Caenorhabditis elegans, T16H12.5. The deduced amino acid sequence had a typical POZ domain and an unidentified region conserved during evolution. No zinc finger or RNA recognition motifs were found in this clone. The 2 kbp mRNA encoding the novel clone SPOP (speckle-type POZ protein) was found to be expressed in all human tissues examined. HA-tagged SPOP, transfected and overexpressed in COS7 cells, exhibited a discrete speckled pattern in the nuclei and was co-localized with the splicing factor, snRNP B′/B. Deletion analysis revealed that both the POZ domain and the evolutionarily conserved region at the amino-terminus are required for the nuclear speckled accumulation of SPOP.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)01340-9