Inhibition of Anchorage-Independent Growth of Tumor Cells by IT-62-B, a New Anthracycline

IT-62-B, a new anthracycline isolated from fermentation broths of Streptomyces sp. IT-62, reversed certain tumor cell phenotypes in vitro including some of human origin. The observed normal phenotypes were anchorage dependence of cell growth, flattened cell morphology and restoration of actin stress...

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Bibliographic Details
Published in:Journal of antibiotics 1997/10/25, Vol.50(10), pp.853-859
Main Authors: TSUCHIYA, KAYOKO S., ISHII, TOMONORI, IKENO, SOUICHI, KUNIMOTO, SETSUKO, KAWAUCHI, TAKASHI, OTANI, TOSHIO, HORI, MAKOTO
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Anthracyclines
Antibiotics, Antineoplastic - pharmacology
Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents
Antineoplastic agents
Applied microbiology
Biological and medical sciences
Cell Division - drug effects
Cell Division - genetics
Chemotherapic agents
DNA Topoisomerases, Type II - metabolism
DNA, Neoplasm - biosynthesis
DNA, Neoplasm - drug effects
Doxorubicin - analogs & derivatives
Doxorubicin - pharmacology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Neoplastic - drug effects
General aspects
Genes, fos
Genes, ras
Humans
Medical sciences
Mice
Microbiology
Mutation
Pharmacology. Drug treatments
Tumor Cells, Cultured
Tumor Stem Cell Assay
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Summary:IT-62-B, a new anthracycline isolated from fermentation broths of Streptomyces sp. IT-62, reversed certain tumor cell phenotypes in vitro including some of human origin. The observed normal phenotypes were anchorage dependence of cell growth, flattened cell morphology and restoration of actin stress fibers. The extent of the anchorage dependence of cell growth induced by IT-62-B was generally greater than that by doxorubicin or pirarubicin. The cell-flattening effect of IT-62-B on cells of T24 (human bladder), but not on C-33A (human cervix), accompanied inhibition of fos gene expression. T24 cells, once flattened by IT-62-B, retained their flat morphology even in drug-free, fresh medium and eventually died in several days. IT-62-B, unlike doxorubicin, only slightly inhibited the topoisomerase II reaction in vitro and DNA synthesis in isolated cell nuclei.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.50.853