Recovery of Replication-Competent HIV Despite Prolonged Suppression of Plasma Viremia

In evaluating current combination drug regimens for treatment of human immunodeficiency virus (HIV) disease, it is important to determine the existence of viral reservoirs. After depletion of CD8 cells from the peripheral blood mononuclear cells (PBMCs) of both patients and normal donors, activation...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1997-11, Vol.278 (5341), p.1291-1295
Main Authors: Wong, Joseph K., Hezareh, Marjan, Gunthard, Huldrych F., Havlir, Diane V., Ignacio, Caroline C., Spina, Celsa A., Richman, Douglas D.
Format: Article
Language:English
Subjects:
AIDS/HIV
Anti-HIV Agents - therapeutic use
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiviral agents
Antivirals
Biological and medical sciences
Blood plasma
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - virology
Coculture Techniques
Combination drug therapy
Cultured cells
Donors
Drug resistance
Drug Resistance, Microbial - genetics
Drug therapy
Drug Therapy, Combination
HIV
HIV (Viruses)
HIV Infections - drug therapy
HIV Infections - virology
HIV-1 - genetics
HIV-1 - isolation & purification
HIV-1 - physiology
Human immunodeficiency virus
Human viral diseases
Humans
Immunologic Memory
Indinavir - therapeutic use
Infectious diseases
Lamivudine - therapeutic use
Lymphocyte Activation
Lymphocytes
Macrophages
Medical research
Medical sciences
Mutation
Patients
Pharmacology. Drug treatments
RNA
RNA, Viral - analysis
RNA, Viral - blood
T lymphocytes
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - virology
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Load
Viremia - drug therapy
Viremia - virology
Virus Activation
Virus Latency
Virus Replication
Viruses
Zidovudine - therapeutic use
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Summary:In evaluating current combination drug regimens for treatment of human immunodeficiency virus (HIV) disease, it is important to determine the existence of viral reservoirs. After depletion of CD8 cells from the peripheral blood mononuclear cells (PBMCs) of both patients and normal donors, activation of patient CD4 lymphocytes with immobilized antibodies to CD3 and CD28 enabled the isolation of virus from PBMCs of six patients despite the suppression of their plasma HIV RNA to fewer than 50 copies per milliliter for up to 2 years. Partial sequencing of HIV pol revealed no new drug resistance mutations or discernible evolution, providing evidence for viral latency rather than drug failure.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.278.5341.1291