Human Papillomavirus Type 11 (HPV-11) Neutralizing Antibodies in the Serum and Genital Mucosal Secretions of African Green Monkeys Immunized with HPV-11 Virus-like Particles Expressed in Yeast

It has been shown previously that immunization of animals with recombinant virus-like particles (VLPs) consisting of the viral capsid proteins L1 or L1 plus L2 protected animals against experimental viral challenge. However, none of these experimental models addresses the issue of whether systemic i...

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Bibliographic Details
Published in:The Journal of infectious diseases 1997-11, Vol.176 (5), p.1141-1145
Main Authors: Lowe, Robert S., Brown, Darron R., Bryan, Janine T., Cook, James C., George, Hugh A., Hofmann, Kathryn J., Hurni, William M., Joyce, Joseph G., Lehman, E. Dale, Markus, Henry Z., Neeper, Michael P., Schultz, Loren D., Shaw, Alan R., Jansen, Kathrin U.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Viral - blood
Biological and medical sciences
Cercopithecus aethiops
Female
Fundamental and applied biological sciences. Psychology
Genitalia
Human papillomavirus 11
Human papillomavirus 16
Immunity, Mucosal
Immunization
Immunoglobulin G - blood
Infections
Major Articles
Microbiology
Papillomaviridae - immunology
Saccharomyces cerevisiae - genetics
Secretion
Therapeutic irrigation
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - immunology
Vagina - immunology
Viral Vaccines - immunology
Virion - immunology
Virology
Viruses
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Summary:It has been shown previously that immunization of animals with recombinant virus-like particles (VLPs) consisting of the viral capsid proteins L1 or L1 plus L2 protected animals against experimental viral challenge. However, none of these experimental models addresses the issue of whether systemic immunization with VLPs elicits a neutralizing antibody response in the genital mucosa. Such a response may be necessary to protect the uterine cervix against infection with genital human papillomavirus (HPV) types. African green monkeys systemically immunized with HPV-11 VLPs expressed in Saccharomyces cerevisiae and formulated on aluminum adjuvant elicited high-titered HPV-11 VLP-specific serum antibody responses. Sera from these immunized monkeys neutralized HPV-11 in the athymic mouse xenograft system. Significant levels of HPV-11-neutralizing antibodies also were observed in cervicovaginal secretions. These findings suggest that protection against HPV infection of the uterine cervix may be possible through systemic immunization with HPV VLPs.
ISSN:0022-1899
1537-6613
DOI:10.1086/514105