Characteristics of an Unusual Anaerobic Pigmented Gram-Negative Rod Isolated from Normal and Inflamed Appendices

During our studies of the bacterial etiology of appendicitis, we often isolated a previously undescribed anaerobic gram-negative rod. This organism resembled the Bacteroides fragilis group because it was resistant to bile and because of its special-potency-disk pattern (resistant to vancomycin, kana...

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Bibliographic Details
Published in:Clinical infectious diseases 1997-09, Vol.25 (Supplement-2), p.S107-S110
Main Authors: Rautio, Merja, Lönnroth, Maarit, Saxén, Harri, Nikku, Risto, Väisänen, Marja-Liisa, Finegold, Sydney M., Somer, Hannele Jousimies
Format: Article
Language:English
Subjects:
Adolescent
Anaerobic bacteria
Appendicitis
Appendicitis - microbiology
Appendix - microbiology
Application programming interfaces
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacteroides
Bacteroides fragilis - isolation & purification
Bile
Bile - physiology
Biological and medical sciences
Blood
Child
Child, Preschool
Fatty acids
Fatty Acids - analysis
Gram-Negative Anaerobic Bacteria - chemistry
Gram-Negative Anaerobic Bacteria - isolation & purification
Human bacterial diseases
Humans
Infant
Infectious diseases
Medical sciences
Pigments, Biological - analysis
Porphyromonas
Prevotella
Taxonomy and Clinical Significance
Writing tablets
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Summary:During our studies of the bacterial etiology of appendicitis, we often isolated a previously undescribed anaerobic gram-negative rod. This organism resembled the Bacteroides fragilis group because it was resistant to bile and because of its special-potency-disk pattern (resistant to vancomycin, kanamycin, and colistin), but unlike the B. fragilis group, this bacterium produced brown pigment on media containing hemolysed blood. The cellular fatty acid pattern, with iso-C15:0 being the predominant acid, was most closely related to the fatty acid profile of Porphyromonas species; however, this organism differed from Porphyromonas species by being bile-resistant and by not producing butyrate as a metabolic endproduct. Enzymatic activities of 31 isolates were determined with use of the API ZYM system and Rosco diagnostic tablets. These profiles were different from those of Prevotella, Porphyromonas, and related species. This organism was isolated from 40% of appendiceal tissue samples; no obvious qualitative or quantitative difference in rates of isolation from patients with inflamed or normal appendices was observed.
ISSN:1058-4838
1537-6591
DOI:10.1086/516210