II. Localization and characterization of dopamine D4 binding sites in rat and human brain by use of the novel, D4 receptor-selective ligand [3H]NGD 94-1

The dopamine D4 selective ligand, [H]NGD 94-1, was used in these studies to characterize binding sites in rat and human brain tissue by membrane binding and autoradiography techniques. Autoradiographic analysis of rat brain showed that specific [3H]NGD 94-1 binding was greatest in entorhinal cortex,...

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Published in:The Journal of pharmacology and experimental therapeutics 1997-08, Vol.282 (2), p.1020-1027
Main Authors: Primus, R J, Thurkauf, A, Xu, J, Yevich, E, McInerney, S, Shaw, K, Tallman, J F, Gallagher, D W
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Brain - metabolism
Dopamine Antagonists - metabolism
Dopamine D2 Receptor Antagonists
Humans
Imidazoles - metabolism
Male
Pyrimidines - metabolism
Raclopride
Radioligand Assay
Rats
Rats, Sprague-Dawley
Receptors, Dopamine D2 - metabolism
Receptors, Dopamine D4
Salicylamides - metabolism
Tetrahydronaphthalenes - metabolism
Tritium
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Summary:The dopamine D4 selective ligand, [H]NGD 94-1, was used in these studies to characterize binding sites in rat and human brain tissue by membrane binding and autoradiography techniques. Autoradiographic analysis of rat brain showed that specific [3H]NGD 94-1 binding was greatest in entorhinal cortex, lateral septal nucleus, hippocampus and the medial preoptic area of the hypothalamus. This nonstriatal distribution of [3H]NGD 94-1 binding was distinct from the autoradiographic distribution of dopamine D2 and D3 receptor subtypes. In homogenate preparations from rat brain regions, [3H]NGD 94-1 binding sites were low in density (
ISSN:0022-3565
1521-0103