Correlation of MDR1 Gene Expression With Chemotherapy in Neuroblastoma

Forty-one neuroblastoma tumor specimens have been analyzed by Northern and slot blot hybridization techniques with human MDR1 gene probes. Only one of 15 (6%) tumors from patients who had not received chemotherapy exhibited high levels of MDR1 transcripts, while 11 of 26 (42%) treated tumors showed...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1989-09, Vol.81 (18), p.1401-1405
Main Authors: Bourhis, Jean, Bénard, Jean, Hartmann, Oliver, Boccon-Gibod, Liliane, Lemerle, Jean, Riou, Guy
Format: Article
Language:English
Subjects:
Adrenal Gland Neoplasms - genetics
Adrenal Gland Neoplasms - therapy
Antineoplastic Agents - therapeutic use
Biological and medical sciences
Blotting, Northern
Bone Marrow - immunology
Combined Modality Therapy
DNA Probes
DNA, Neoplasm - analysis
Humans
Medical sciences
Neuroblastoma - genetics
Neuroblastoma - secondary
Neuroblastoma - therapy
Neurology
RNA, Messenger - analysis
RNA, Neoplasm - analysis
Transcription Factors - analysis
Tumor Cells, Cultured
Tumors of the nervous system. Phacomatoses
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Summary:Forty-one neuroblastoma tumor specimens have been analyzed by Northern and slot blot hybridization techniques with human MDR1 gene probes. Only one of 15 (6%) tumors from patients who had not received chemotherapy exhibited high levels of MDR1 transcripts, while 11 of 26 (42%) treated tumors showed high levels of MDR1 expression (Fisher exact test: P=.03). The results indicate that the level of MDR1 mRNA expression is associated with previous chemotherapy, including drugs that select the multidrug resistance phenotype in vitro regardless of neuroblastoma tissue origin or N-myc content in the genome. For the 26 treated neuroblastomas, the number of nonresponsive tumors was found to be significantly higher among those with high levels of MDR1 mRNA. [J NatI Cancer Inst 81: 1401–1405, 1989]
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/81.18.1401