Molecular expression of 17 βhydroxysteroid dehydrogenase types in relation to their activity in intact human prostate cancer cells

In the present study we have inspected estrogen metabolism in cultured human prostate cancer cells (LNCaP, DU145, PC3), in relation to the expression of mRNAs for different 17 βhydroxysteroid dehydrogenase (17 βHSD) enzymes (from 1 to 4). Using an intact cell analysis, we have compared precursor deg...

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Published in:Molecular and cellular endocrinology 1997-07, Vol.131 (1), p.51-57
Main Authors: Carruba, Giuseppe, Adamski, Jerzy, Calabrò, Maurizio, Miceli, M.Dora, Cataliotti, Antonella, Bellavia, Vincenzo, Lo Bue, Arianna, Polito, Lucia, Castagnetta, Luigi A
Format: Article
Language:English
Subjects:
17 βhydroxysteroid dehydrogenase
17-Hydroxysteroid Dehydrogenases - chemistry
17-Hydroxysteroid Dehydrogenases - genetics
17-Hydroxysteroid Dehydrogenases - metabolism
Enzyme Activation - genetics
estrogen metabolism
Estrogens - metabolism
Estrogens, Conjugated (USP) - metabolism
Humans
Male
prostate cancer
Prostatic Neoplasms - enzymology
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
RNA, Messenger - biosynthesis
Tumor Cells, Cultured
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Summary:In the present study we have inspected estrogen metabolism in cultured human prostate cancer cells (LNCaP, DU145, PC3), in relation to the expression of mRNAs for different 17 βhydroxysteroid dehydrogenase (17 βHSD) enzymes (from 1 to 4). Using an intact cell analysis, we have compared precursor degradation and product formation after incubation of cells with physiological amounts of radioactive E 2 or estrone (E 1) for 24–72 h and subsequent reverse-phase high performance liquid chromatography analysis. The LNCaP and DU145 cells only partly converted E 2 to E 1 (26 and 13% at 72 h, respectively), giving rise to an appreciable production of E 2 from E 1 (nearly 20% in all cases). Conversely, PC3 cells revealed a massive E 2 oxidation to E 1 (up to 90% by 72 h) and a scant formation of E 2 (
ISSN:0303-7207
1872-8057
DOI:10.1016/S0303-7207(97)00092-0