Human fibroblast growth factor receptor 3 gene (FGFR3) : genomic sequence and primer set information for gene analysis

We have determined the nucleotide sequence of the human fibroblast growth factor receptor 3 (FGFR3) gene, including 800 bp of the 5'-flanking region and compared the sequence with the previously published murine Fgfr3 gene. The organization of the gene is highly conserved between man and mouse....

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Bibliographic Details
Published in:Human genetics 1997-08, Vol.100 (2), p.215-219
Main Authors: WÜCHNER, C, HILBERT, K, ZABEL, B, WINTERPACHT, A
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA Primers
Exons
Fundamental and applied biological sciences. Psychology
Genes. Genome
Genomic Library
Humans
Introns
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Polymerase Chain Reaction
Protein-Tyrosine Kinases
Receptor, Fibroblast Growth Factor, Type 3
Receptors, Fibroblast Growth Factor - genetics
RNA Splicing
Sequence Analysis, DNA
Sequence Homology
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Summary:We have determined the nucleotide sequence of the human fibroblast growth factor receptor 3 (FGFR3) gene, including 800 bp of the 5'-flanking region and compared the sequence with the previously published murine Fgfr3 gene. The organization of the gene is highly conserved between man and mouse. We used the intron sequences to design a set of primers that allow amplification of the 17 exons (2-18) that encode the complete open reading frame. Using these primers the FGFR3 gene can be amplified at the genomic level, which significantly facilitates mutational screening.
ISSN:0340-6717
1432-1203
DOI:10.1007/s004390050493