Incidence of anti-DNA idiotype-positive cells in human peripheral blood

Human anti-DNA idiotype (Id)-bearing cells in peripheral blood were sought using mouse monoclonal anti-Id antibodies to human monoclonal anti-DNA antibodies. Pretreatment with acid pH or pronase P or preincubation in human serum-free medium markedly decreased the number of anti-Id-reactive cells. Pr...

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Bibliographic Details
Published in:Journal of clinical immunology 1989-07, Vol.9 (4), p.313-321
Main Authors: Takai, O, Sasaki, T, Muryoi, T, Harata, N, Hashimoto, H, Yoshinaga, K
Format: Article
Language:English
Subjects:
Antibodies, Antinuclear - analysis
Antibody-Producing Cells - analysis
B-Lymphocytes - immunology
DNA - immunology
Humans
Immunoglobulin Idiotypes
Lupus Erythematosus, Systemic - immunology
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Summary:Human anti-DNA idiotype (Id)-bearing cells in peripheral blood were sought using mouse monoclonal anti-Id antibodies to human monoclonal anti-DNA antibodies. Pretreatment with acid pH or pronase P or preincubation in human serum-free medium markedly decreased the number of anti-Id-reactive cells. Pronase P-treated cells were able to bind to anti-Id once more after incubation for 18 hr at 37 degrees C, indicating the resynthesis of internal idiotypic determinants on the cells. Antiidiotype-reactive cells retained the same idiotypes in their cytoplasma. The cells expressing anti-DNA idiotypes, termed 0-81 and NE-1, were detected in the circulation of most patients with active lupus nephritis but not in those of inactive systemic lupus erythematosus (SLE) and healthy subjects. The idiotype-positive cells occurred in up to 5-10% of B cells from some patients with SLE in the active stage but became undetectable in remission. A limited number of anti-DNA Id-positive cells responsible for anti-DNA production might be preferentially expanded during acute episodes of the disease in some patients with SLE.
ISSN:0271-9142
1573-2592
DOI:10.1007/bf00918663