Mitosis in living budding yeast: anaphase a but no metaphase plate

Chromosome movements and spindle dynamics were visualized in living cells of the budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by expression of a protein fusion between green fluorescent protein (GFP) and the Lac repressor, which bound to an array of Lac operator...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1997-07, Vol.277 (5325), p.574-578
Main Authors: Straight, A.F. (University of California, San Francisco, CA.), Marshall, W.F, Sedat, J.W, Murray, A.W
Format: Article
Language:English
Subjects:
Anaphase
Animals
Bacterial Proteins - metabolism
Biochemistry
Biological and medical sciences
CELL DIVISION
Centromere - chemistry
Centromere - physiology
Centromeres
Chromatids
Chromatids - physiology
CHROMOSOME
CHROMOSOMES
Chromosomes, Fungal - chemistry
Chromosomes, Fungal - physiology
CROMOSOMAS
Cytogenetics
DIVISION CELLULAIRE
DIVISION CELULAR
Escherichia coli Proteins
Exhibits
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Green Fluorescent Proteins
Invertebrata
Kinetics
Kinetochores
Lac Repressors
Luminescent Proteins
MEIOSE
MEIOSIS
Metaphase
Microscopy, Fluorescence
Microtubules
Microtubules - ultrastructure
Mitosis
Mitotic spindle apparatus
Movement
Observation
Operator Regions, Genetic
Recombinant Fusion Proteins
Repressor Proteins - metabolism
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - cytology
Spindle Apparatus - physiology
Spindle Apparatus - ultrastructure
Telomere - physiology
Telomeres
Tubulin - analysis
Yeast
Yeasts
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Summary:Chromosome movements and spindle dynamics were visualized in living cells of the budding yeast Saccharomyces cerevisiae. Individual chromosomal loci were detected by expression of a protein fusion between green fluorescent protein (GFP) and the Lac repressor, which bound to an array of Lac operator binding sites integrated into the on chromosome. Spindle microtubules were detected by expression of a protein fusion between GFP and Tub1, the major alpha tubulin. Spindle elongation and chromosome separation exhibited biphasic kinetics, and centromeres separated before telomeres. Budding yeast did not exhibit a conventional metaphase chromosome alignment but did show anaphase A, movement of the chromosomes to the poles
ISSN:0036-8075
1095-9203
DOI:10.1126/science.277.5325.574