Sorbitol accumulation and transmembrane efflux in osmotically stressed JS1 schwannoma cells

Sorbitol accumulation and transmembrane efflux in JS1 schwannoma cells were examined during osmotic stress in the presence of a sorbitol dehydrogenase (SD) inhibitor and following return to iso-osmotic conditions. SD inhibition promoted sorbitol accumulation under hyperglycemic and/or hyperosmotic c...

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Bibliographic Details
Published in:Neuroscience letters 1997-06, Vol.229 (1), p.53-56
Main Authors: Mizisin, Andrew P, Li, Li, Calcutt, Nigel A
Format: Article
Language:English
Subjects:
Aldose reductase
Animals
Biological and medical sciences
Brain Neoplasms - metabolism
Cell culture
Cell Membrane - metabolism
Culture Media
Fundamental and applied biological sciences. Psychology
Isolated neuron and nerve. Neuroglia
L-Iditol 2-Dehydrogenase - antagonists & inhibitors
L-Iditol 2-Dehydrogenase - metabolism
Neurilemmoma - metabolism
Osmoregulation
Osmotic Pressure
Polymers - metabolism
Polyol
Quinidine - pharmacology
Rats
Schwann cells
Sorbitol - metabolism
Sorbitol dehydrogenase
Sorbitol pathway
Temperature
Transmembrane efflux
Tumor Cells, Cultured
Vertebrates: nervous system and sense organs
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Summary:Sorbitol accumulation and transmembrane efflux in JS1 schwannoma cells were examined during osmotic stress in the presence of a sorbitol dehydrogenase (SD) inhibitor and following return to iso-osmotic conditions. SD inhibition promoted sorbitol accumulation under hyperglycemic and/or hyperosmotic conditions, and sorbitol efflux during iso-osmotic incubation was prevented by cooling to 4°C or by quinidine. It appears that sorbitol levels in JS1 cells are dependent on SD activity and that sorbitol is rapidly removed upon restoring iso-osmotic conditions.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(97)00416-3