Microvascular Endothelial Cell Culture

Microvascular endothelial cells play a central role in inflammation, tumor metastasis, and wound healing. Methods to study these processes in vitro using cells isolated from adult skin, from the inner and the outer segments of the neontal foreskin, and from experimental animals are reviewed. A new m...

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Bibliographic Details
Published in:Journal of investigative dermatology 1989-08, Vol.93 (2), p.S33-S38
Main Author: Karasek, Marvin A.
Format: Article
Language:English
Subjects:
Adult
Animals
Cell Division
Cell Separation - methods
Cells, Cultured
Cytological Techniques
Endothelium, Vascular - cytology
Endothelium, Vascular - physiology
Endothelium, Vascular - ultrastructure
Humans
Infant, Newborn
Male
Microcirculation
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Summary:Microvascular endothelial cells play a central role in inflammation, tumor metastasis, and wound healing. Methods to study these processes in vitro using cells isolated from adult skin, from the inner and the outer segments of the neontal foreskin, and from experimental animals are reviewed. A new modified Iscove's medium supplemented with 2% prepartum maternal serum, dibutyryl cyclic AMP, isobutyl methylxanthine, thymidine, and hypoxanthine is described. This modified medium supports growth of both adult and neonatal endothelial cells up to seven passages with retention of Cytologic markers closely identified with endothelial cells (Weibel-Palade bodies, Factor VIII-associated antigen). Several functions associated with the microvasculature in situ are expressed by microvascular endothelial cells in cell culture. Such functions include the formation of a basement membrane, angiogenesis, intercelluar gap formation in response to vasoactive agents, and the attachment and migration of lymphocytes through endothelial monolayers. J Invest Dermatol 93:33S–38S, 1989
ISSN:0022-202X
1523-1747
DOI:10.1038/jid.1989.6