Simple approach for analysis of plasma oxo-, hydroxy- and dicarboxylic acids

Following deproteinization of plasma with organic solvents the supernatant was shaken with hexane and cation-exchange resin in an Eppendorf tube to remove fatty and amino acids and the medium was subjected to direct treatment with ethyl chloroformate under catalytic influence of pyridine. A subseque...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-06, Vol.693 (2), p.499-502
Main Authors: Hušek, Petr, Matucha, Petr
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carboxylic acids
Chromatography, Gas
Dicarboxylic acids
Dicarboxylic Acids - blood
Formic Acid Esters
Humans
Hydroxy Acids - blood
Hydroxycarboxylic acids
Investigative techniques, diagnostic techniques (general aspects)
Keto Acids - blood
Ketocarboxylic acids
Medical sciences
Metabolic diseases
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reproducibility of Results
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Summary:Following deproteinization of plasma with organic solvents the supernatant was shaken with hexane and cation-exchange resin in an Eppendorf tube to remove fatty and amino acids and the medium was subjected to direct treatment with ethyl chloroformate under catalytic influence of pyridine. A subsequent extraction of the immediately formed ethyl esters with a drop of chloroform enabled us to subject the sample to gas chromatographic (GC) analysis. Since ketocarboxylic acids do not require a preliminary oximation the total time of sample workup and analysis takes only several minutes.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(97)00026-1