Expression in anthers of two genes encoding Brassica oleracea transmembrane channel proteins

Screening of an anther cDNA expression library resulted in the isolation of two almost identical cDNA clones, termed mipA and mipB, showing homology with sequences encoding transmembrane channel proteins from the MIP family. Both clones were expressed in several tissues, but not in pollen. MipA was...

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Bibliographic Details
Published in:Plant molecular biology 1997-05, Vol.34 (1), p.163-168
Main Authors: Ruiter, R.K. (Nijmegen Univ. (Netherlands). Dept. of Experimental Botany), Eldik, G.J. van, Herpen, M.M.A. van, Schrauwen, J.A.M, Wullems, G.J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
ANTERA
ANTHERE
ANTHERS
Brassica - genetics
BRASSICA OLERACEA
CODE GENETIQUE
CODIGO GENETICO
EXPRESION GENICA
EXPRESSION DES GENES
GENE EXPRESSION
Gene Expression Regulation, Plant
Genes, Plant
GENETIC CODE
Ion Channels - biosynthesis
Ion Channels - genetics
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Plant Proteins - biosynthesis
Plant Proteins - genetics
POLEN
POLLEN
Pollen - chemistry
Pollen - genetics
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
TRANSCRIPCION
TRANSCRIPTION
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Summary:Screening of an anther cDNA expression library resulted in the isolation of two almost identical cDNA clones, termed mipA and mipB, showing homology with sequences encoding transmembrane channel proteins from the MIP family. Both clones were expressed in several tissues, but not in pollen. MipA was preferentially expressed in the surrounding sporophytic tissues of stamens. Anthers subjected to drought were induced to accumulate even more mip transcripts, which was entirely due to higher mipA gene expression. On basis of isolation procedures, sequence homology and drought inducibility of mipA we conclude that the encoded proteins probably are constituents of the pollen coat and are aquaporins.
ISSN:0167-4412
1573-5028
DOI:10.1023/A:1005828325425