Determination of Esterase-Catalyzed Cocaine Metabolite Formation by High-Performance Liquid Chromatography with Ultraviolet Detection

A reversed-phase high-performance liquid chromatographic (HPLC) method for the determination of cocaine metabolites produced in vitro by serum and liver esterases is described. Hydrolysis of cocaine at the methyl ester bond produces benzoylecgonine and methanol, whereas hydrolysis at the benzoyl est...

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Bibliographic Details
Published in:Journal of chromatographic science 1997-06, Vol.35 (6), p.287-290
Main Authors: Kim, Kelly Y., Bornheim, Lester M.
Format: Article
Language:English
Subjects:
Animals
Benzoates - analysis
Benzoates - blood
Benzoates - urine
Benzoic Acid
Biological and medical sciences
Biotransformation
Calibration
Chromatography, High Pressure Liquid
Cocaine - analogs & derivatives
Cocaine - analysis
Cocaine - blood
Cocaine - pharmacokinetics
Cocaine - urine
Cytosol - enzymology
Cytosol - metabolism
Drug addictions
Esterases - metabolism
In Vitro Techniques
Male
Medical sciences
Mice
Mice, Inbred Strains
Microsomes, Liver - enzymology
Microsomes, Liver - metabolism
Narcotics - pharmacokinetics
Spectrophotometry, Ultraviolet
Toxicology
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Summary:A reversed-phase high-performance liquid chromatographic (HPLC) method for the determination of cocaine metabolites produced in vitro by serum and liver esterases is described. Hydrolysis of cocaine at the methyl ester bond produces benzoylecgonine and methanol, whereas hydrolysis at the benzoyl ester bond produces ecgonine methyl ester and benzoic acid. This method quantitates benzoic acid (as a measure of ecgonine methyl ester formation) and benzoylecgonine, which can be determined simultaneously and with great sensitivity by HPLC and ultraviolet detection. Cocaine is found to be hydrolyzed to both ecgonine methyl ester and benzoylecgonine; hepatic microsomes exhibit the highest specific activity.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/35.6.287