Pharmacological effects of nematode FMRFamide-related peptides (FaRPs) on muscle contractility of the trematode, Fasciola hepatica

The physiological effects of synthetic replicates of the nematode FaRPs, AF1 (KNEFIRFamide), AF2 (KHEYLRFamide), PF1 (SDPNFLRFamide), PF2 (SADPNFLRFamide), AF8/PF3 (KSAYMRFamide) and PF4 (KPNFIRFamide) were examined on muscle preparations of the liver fluke, Fasciola hepatica. Changes in contractili...

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Bibliographic Details
Published in:Parasitology 1997-06, Vol.114 (6), p.531-539
Main Authors: MARKS, N. J., MAULE, A. G., HALTON, D. W., GEARY, T. G., SHAW, C., THOMPSON, D. P.
Format: Article
Language:English
Subjects:
Animals
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Electrophysiology
FaRPs
Fasciola hepatica
Fasciola hepatica - drug effects
Fasciola hepatica - physiology
FMRFamide
FMRFamide-related peptides
Fundamental and applied biological sciences. Psychology
Invertebrates
Muscle Contraction - drug effects
Nemathelminthia. Plathelmintha
Nematoda - chemistry
nematode peptides
neuromuscular activity
Neuropeptides - chemical synthesis
Neuropeptides - chemistry
Neuropeptides - pharmacology
Oligopeptides - pharmacology
physiology
Physiology. Development
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Summary:The physiological effects of synthetic replicates of the nematode FaRPs, AF1 (KNEFIRFamide), AF2 (KHEYLRFamide), PF1 (SDPNFLRFamide), PF2 (SADPNFLRFamide), AF8/PF3 (KSAYMRFamide) and PF4 (KPNFIRFamide) were examined on muscle preparations of the liver fluke, Fasciola hepatica. Changes in contractility following the addition of the test compound were recorded using a photo-optic transducer system. Unlike the varied effects these peptides have on nematode somatic musculature, all were found to induce excitatory responses in the muscle activity of F. hepatica. While qualitative effects of the nematode peptides were similar in that they induced increases in both the amplitude and frequency of F. hepatica muscle contractions, they varied considerably in the potency of their excitatory effects. The threshold activity for each peptide was as follows: 10 μM, PF1 and PF2; 3 μM, AF1 and PF3; 1 μM, AF2; and 30 nM, PF4. The results demonstrate, for the first time, the cross-phyla activity of nematode neuropeptides on the neuromuscular activity of a trematode.
ISSN:0031-1820
1469-8161
DOI:10.1017/S003118209700886X