Structure and function of the androgen receptor

The androgen receptor in several species (human, rat, calf) is a monomeric protein with a molecular mass of 100-110 kDa. The steroid binding domain is confined to a region of 30 kDa, while the DNA-binding domain has the size of approx. 10 kDa. A 40 kDa fragment containing both the DNA and steroid bi...

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Bibliographic Details
Published in:Urological research 1989-03, Vol.17 (2), p.87-93
Main Authors: Brinkmann, A O, Klaasen, P, Kuiper, G G, van der Korput, J A, Bolt, J, de Boer, W, Smit, A, Faber, P W, van Rooij, H C, Geurts van Kessel, A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Binding Sites
Cattle
Cloning, Molecular
DNA - genetics
DNA - metabolism
Female
Humans
In Vitro Techniques
Kinetics
Male
Molecular Sequence Data
Molecular Structure
Molecular Weight
Rats
Receptors, Androgen - genetics
Receptors, Androgen - metabolism
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Summary:The androgen receptor in several species (human, rat, calf) is a monomeric protein with a molecular mass of 100-110 kDa. The steroid binding domain is confined to a region of 30 kDa, while the DNA-binding domain has the size of approx. 10 kDa. A 40 kDa fragment containing both the DNA and steroid binding domain displayed a higher DNA binding activity than did the intact 100 kDa molecule. cDNA encoding the major part of the human androgen receptor was isolated. The cDNA contains an open reading frame of 2,277 bp but still lacks part of the 5'-coding sequence. Homology with the progesterone and glucocorticoid receptor was about 80% in the DNA binding domain and 50% in the steroid binding domain. The present data provide evidence that the androgen receptor belongs to the superfamily of ligand responsive transcriptional regulators and consists of three distinct domains each with a specialized function.
ISSN:0300-5623
1434-0879
DOI:10.1007/BF00262026