Crystallization and preliminary X-ray diffraction studies of oxidized flavodoxin from Chondrus crispus, a red alga

Crystals of the oxidized form of flavodoxin from a red alga, Chondrus crispus, have been grown in ammonium sulfate solution by the dialysis method. The crystals belong to the orthorhombic system, space group P212121, with unit cell dimensions of a=63.6, b=48.8, and c=56.8 Å. The asymmetric unit cont...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1989, Vol.105 (3), p.348-350
Main Authors: Fukuyama, K, Matsubara, H, Katsube, Y, Rogers, L.J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chondrus crispus
Crystalline structure
Crystallization
flavodoxin
Flavodoxin - analysis
Flavoproteins - analysis
Fundamental and applied biological sciences. Psychology
Molecular biophysics
Oxidation-Reduction
oxidoreductases
polypeptides
proteins
Rhodophyta - analysis
Structure in molecular biology
X-Ray Diffraction
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Summary:Crystals of the oxidized form of flavodoxin from a red alga, Chondrus crispus, have been grown in ammonium sulfate solution by the dialysis method. The crystals belong to the orthorhombic system, space group P212121, with unit cell dimensions of a=63.6, b=48.8, and c=56.8 Å. The asymmetric unit contains one molecule of flavodoxin. The crystals diffract X-rays to about 2.0 Å resolution and are stable to X-ray beams. The diffraction patterns changed significantly upon soaking the crystal in a solution of a platinum complex. The major heavy-atom sites in the platinum derivative crystal have been identified from the difference Patterson function calculated at 4 Å resolution.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a122666