High sensitivity dry rehydratable film method for enumeration of coliforms in dairy products: collaborative study

A dry-film coliform count plate that is inoculated with 5 mL sample was compared with the Violet Red Bile Agar plate method in a collaborative study by 18 laboratories. Products analyzed were 2% milk, chocolate milk, cream, vanilla ice cream, cottage cheese, and cheese. Collaborators tested blind du...

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Bibliographic Details
Published in:Journal of AOAC International 1997-05, Vol.80 (3), p.505-516
Main Authors: Curiale, M.S. (Silliker Laboratories Group, Inc., South Holland, IL.), Gangar, V, D'onorio, A, Gambrel-Lenarz, S, McAllister, J.S
Format: Article
Language:English
Subjects:
Agar
ALIMENTOS
ANALISIS BIOLOGICO
ANALYSE BIOLOGIQUE
Animals
BACTERIA COLIFORME
BACTERIE COLIFORME
Bacteriological Techniques
Biological and medical sciences
Cheese - microbiology
Colony Count, Microbial - instrumentation
Colony Count, Microbial - methods
CONTAMINACION
CONTAMINACION BIOLOGICA
CONTAMINATION
CONTAMINATION BIOLOGIQUE
Dairy Products - microbiology
Enterobacteriaceae - isolation & purification
Food industries
Fundamental and applied biological sciences. Psychology
Ice Cream - microbiology
Milk - microbiology
Milk and cheese industries. Ice creams
PRODUCTOS LACTEOS
PRODUIT ALIMENTAIRE
PRODUIT LAITIER
Water
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Summary:A dry-film coliform count plate that is inoculated with 5 mL sample was compared with the Violet Red Bile Agar plate method in a collaborative study by 18 laboratories. Products analyzed were 2% milk, chocolate milk, cream, vanilla ice cream, cottage cheese, and cheese. Collaborators tested blind duplicate uninoculated samples and samples inoculated at low, medium, and high level. Significantly (P 0.05) higher numbers of coliforms were recovered by the dry-film method from 2% milk samples at the 3 inoculum levels, the chocolate milk at the low- and high-inoculum levels, and the cream at the high-inoculum level. Significantly higher counts were obtained by the agar method for cottage cheese samples at the low-inoculum level. The repeatability standard deviation for the dry-film method was significantly higher for the high-inoculum level chocolate milk sample and the medium-inoculum level cottage cheese. The same statistic was significantly higher for the agar method at all 3 inoculum levels in the 2% milk and the medium-inoculum level cream. The high-sensitivity dry rehydratable film method for enumeration of coliforms in dairy products has been adopted first action by AOAC INTERNATIONAL
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/80.3.505