Migratory properties of skin dendritic cells of the rat

Dendritic cells (DC), in the epidermis identified as Langerhans cells (LC), are crucial in the initiation of immune responses. After allogeneic skin transplantation, LC migrating from the graft to the draining lymph node of the recipient are supposed to play an important role in the initiation of sk...

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Bibliographic Details
Published in:Transplantation proceedings 1997-05, Vol.29 (3), p.1745-1747
Main Authors: Richters, C.D., van Gelderop, E., du Pont, J.S., Hoekstra, M.J., Kamperdijk, E.W.A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Movement
Cells, Cultured
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocompatibility Antigens Class I - analysis
Leukocyte Common Antigens - immunology
Lymph Nodes - immunology
Lymph Nodes - physiology
Macrophages - cytology
Macrophages - immunology
Male
Rats
Rats, Inbred ACI
Rats, Inbred Strains
Skin - immunology
Tissue, organ and graft immunology
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Summary:Dendritic cells (DC), in the epidermis identified as Langerhans cells (LC), are crucial in the initiation of immune responses. After allogeneic skin transplantation, LC migrating from the graft to the draining lymph node of the recipient are supposed to play an important role in the initiation of skin rejection.' In earlier studies, we observed that during culture of rat skin explants, antigen-presenting DCs including LCs migrate “spontaneously” into the medium. 2,3 To obtain more insight in the migratory properties of these in vitro migrating skin DC, we injected them subcutaneously in the hind footpads of congeneic and allogeneic recipient rats. To trace migrating cells in sections of the draining popliteal lymph node, we used the rat strain PVG-RT7b as donor rats and, as recipients, the congeneic PVG and the allogeneic ACI rats. The leukocytes of the PVG-RT7b bear an epitope that can be recognized using the monoclonal antibody His 41. 4 Using this model, we compared the migration patterns of the injected cells in the congeneic lymph node with those of the allogeneic.
ISSN:0041-1345
1873-2623
DOI:10.1016/S0041-1345(97)00040-7