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Permselectivity of Angiogenic Microvessels Following Alteration of the Endothelial Fiber Matrix by Oligosaccharides
The endothelial glycocalyx, which is composed of integral and peripheral glycoconjugates, forms a fibrous matrix that confers macromolecular sieving properties on the microvascular wall. Changes in pore size within the matrix may regulate macromolecular access to the paracellular and/or vesicular tr...
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| Published in: | Microvascular research 1997-03, Vol.53 (2), p.150-155 |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c370t-e7b53272f5436196c6d516f2e5fec25ccecca7568d613b578036f054358d58fc3 |
| container_end_page | 155 |
| container_issue | 2 |
| container_start_page | 150 |
| container_title | Microvascular research |
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| creator | Henry, Charmaine B.S. Durán, Walter N. DeFouw, David O. |
| description | The endothelial glycocalyx, which is composed of integral and peripheral glycoconjugates, forms a fibrous matrix that confers macromolecular sieving properties on the microvascular wall. Changes in pore size within the matrix may regulate macromolecular access to the paracellular and/or vesicular transendothelial pathways. We tested the hypothesis that modifications of the endothelial glycocalyx might play a role in the ontogeny of endothelial permselectivity in proliferating microvessels of the chick chorioallantoic membrane (CAM). Accordingly, we evaluated the effects ofDolichos biflorusagglutinin (DBA) orArachis hypogaeaagglutinin (PNA) lectin binding, andN′N′diacetylchitobiose or hydroxyethyl starch polysaccharide (HES) incorporation on CAM endothelial restriction of FITC-dextrans 40 or 150 at Days 4.5 and 5.0 of development. Extravasation of FITC-dextrans was determined by recording their perivascular interstitial intensities. Following DBA, PNA, andN′N′diacetylchitobiose administration, interstitial accumulation of the tracers near first-order pre- and postcapillaries, and surrounding the capillaries, was similar to that of controls at both Days 4.5 and 5.0. At Day 4.5, pretreatment with HES significantly decreased extravasation of FITC-dextran 40. Thus, retention of HES molecules within the glycocalyx might tighten the matrix, and reduce access of dextran 40 to transendothelial pathways across the angiogenic microvessels. |
| doi_str_mv | 10.1006/mvre.1996.1996 |
| format | article |
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Psychology ; Glycocalyx - physiology ; Polysaccharides - pharmacology ; Porosity ; Vertebrates: cardiovascular system</subject><ispartof>Microvascular research, 1997-03, Vol.53 (2), p.150-155</ispartof><rights>1997 Academic Press</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-e7b53272f5436196c6d516f2e5fec25ccecca7568d613b578036f054358d58fc3</citedby><cites>FETCH-LOGICAL-c370t-e7b53272f5436196c6d516f2e5fec25ccecca7568d613b578036f054358d58fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2648565$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9143546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Henry, Charmaine B.S.</creatorcontrib><creatorcontrib>Durán, Walter N.</creatorcontrib><creatorcontrib>DeFouw, David O.</creatorcontrib><title>Permselectivity of Angiogenic Microvessels Following Alteration of the Endothelial Fiber Matrix by Oligosaccharides</title><title>Microvascular research</title><addtitle>Microvasc Res</addtitle><description>The endothelial glycocalyx, which is composed of integral and peripheral glycoconjugates, forms a fibrous matrix that confers macromolecular sieving properties on the microvascular wall. Changes in pore size within the matrix may regulate macromolecular access to the paracellular and/or vesicular transendothelial pathways. We tested the hypothesis that modifications of the endothelial glycocalyx might play a role in the ontogeny of endothelial permselectivity in proliferating microvessels of the chick chorioallantoic membrane (CAM). Accordingly, we evaluated the effects ofDolichos biflorusagglutinin (DBA) orArachis hypogaeaagglutinin (PNA) lectin binding, andN′N′diacetylchitobiose or hydroxyethyl starch polysaccharide (HES) incorporation on CAM endothelial restriction of FITC-dextrans 40 or 150 at Days 4.5 and 5.0 of development. Extravasation of FITC-dextrans was determined by recording their perivascular interstitial intensities. Following DBA, PNA, andN′N′diacetylchitobiose administration, interstitial accumulation of the tracers near first-order pre- and postcapillaries, and surrounding the capillaries, was similar to that of controls at both Days 4.5 and 5.0. At Day 4.5, pretreatment with HES significantly decreased extravasation of FITC-dextran 40. Thus, retention of HES molecules within the glycocalyx might tighten the matrix, and reduce access of dextran 40 to transendothelial pathways across the angiogenic microvessels.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood vessels and receptors</subject><subject>Capillary Permeability - physiology</subject><subject>Chick Embryo</subject><subject>Dextrans</subject><subject>Disaccharides - pharmacology</subject><subject>Extravasation of Diagnostic and Therapeutic Materials</subject><subject>Fluorescein-5-isothiocyanate - analogs & derivatives</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycocalyx - physiology</subject><subject>Polysaccharides - pharmacology</subject><subject>Porosity</subject><subject>Vertebrates: cardiovascular system</subject><issn>0026-2862</issn><issn>1095-9319</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp1kcFuEzEQQC0EKmnhyg3JB8Rtg-2NvfYxqhqK1Koc4Gx5veN0kHdd7E0gf4_TRL1xmTnMm9HMG0I-cLbkjKkv4z7DkhujnsMrsuDMyMa03LwmC8aEaoRW4i25LOUXY5xLIy7IheGrVq7UgpTvkMcCEfyMe5wPNAW6nraYtjChp_foc9pDqUShmxRj-oPTlq7jDNnNmKYjPz8CvZmGVHNEF-kGe8j03s0Z_9L-QB8iblNx3j-6jAOUd-RNcLHA-3O-Ij83Nz-ub5u7h6_frtd3jW87NjfQ9bIVnQhy1SpulFeD5CoIkAG8kN6D966TSg-Kt73sNGtVYBWWepA6-PaKfD7Nfcrp9w7KbEcsHmJ0E6RdsZ02nVJMV3B5AuuxpWQI9inj6PLBcmaPlu3Rsj0Kfg614eN58q4fYXjBz1pr_dO57op3MWQ3eSwvmFArLZWsmD5h1S7sEbItHmHyMGCuD7FDwv9t8A8AMpqq</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>Henry, Charmaine B.S.</creator><creator>Durán, Walter N.</creator><creator>DeFouw, David O.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970301</creationdate><title>Permselectivity of Angiogenic Microvessels Following Alteration of the Endothelial Fiber Matrix by Oligosaccharides</title><author>Henry, Charmaine B.S. ; Durán, Walter N. ; DeFouw, David O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-e7b53272f5436196c6d516f2e5fec25ccecca7568d613b578036f054358d58fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood vessels and receptors</topic><topic>Capillary Permeability - physiology</topic><topic>Chick Embryo</topic><topic>Dextrans</topic><topic>Disaccharides - pharmacology</topic><topic>Extravasation of Diagnostic and Therapeutic Materials</topic><topic>Fluorescein-5-isothiocyanate - analogs & derivatives</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycocalyx - physiology</topic><topic>Polysaccharides - pharmacology</topic><topic>Porosity</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Henry, Charmaine B.S.</creatorcontrib><creatorcontrib>Durán, Walter N.</creatorcontrib><creatorcontrib>DeFouw, David O.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microvascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Henry, Charmaine B.S.</au><au>Durán, Walter N.</au><au>DeFouw, David O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Permselectivity of Angiogenic Microvessels Following Alteration of the Endothelial Fiber Matrix by Oligosaccharides</atitle><jtitle>Microvascular research</jtitle><addtitle>Microvasc Res</addtitle><date>1997-03-01</date><risdate>1997</risdate><volume>53</volume><issue>2</issue><spage>150</spage><epage>155</epage><pages>150-155</pages><issn>0026-2862</issn><eissn>1095-9319</eissn><coden>MIVRA6</coden><abstract>The endothelial glycocalyx, which is composed of integral and peripheral glycoconjugates, forms a fibrous matrix that confers macromolecular sieving properties on the microvascular wall. Changes in pore size within the matrix may regulate macromolecular access to the paracellular and/or vesicular transendothelial pathways. We tested the hypothesis that modifications of the endothelial glycocalyx might play a role in the ontogeny of endothelial permselectivity in proliferating microvessels of the chick chorioallantoic membrane (CAM). Accordingly, we evaluated the effects ofDolichos biflorusagglutinin (DBA) orArachis hypogaeaagglutinin (PNA) lectin binding, andN′N′diacetylchitobiose or hydroxyethyl starch polysaccharide (HES) incorporation on CAM endothelial restriction of FITC-dextrans 40 or 150 at Days 4.5 and 5.0 of development. Extravasation of FITC-dextrans was determined by recording their perivascular interstitial intensities. Following DBA, PNA, andN′N′diacetylchitobiose administration, interstitial accumulation of the tracers near first-order pre- and postcapillaries, and surrounding the capillaries, was similar to that of controls at both Days 4.5 and 5.0. At Day 4.5, pretreatment with HES significantly decreased extravasation of FITC-dextran 40. Thus, retention of HES molecules within the glycocalyx might tighten the matrix, and reduce access of dextran 40 to transendothelial pathways across the angiogenic microvessels.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>9143546</pmid><doi>10.1006/mvre.1996.1996</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-2862 |
| ispartof | Microvascular research, 1997-03, Vol.53 (2), p.150-155 |
| issn | 0026-2862 1095-9319 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Animals Biological and medical sciences Blood vessels and receptors Capillary Permeability - physiology Chick Embryo Dextrans Disaccharides - pharmacology Extravasation of Diagnostic and Therapeutic Materials Fluorescein-5-isothiocyanate - analogs & derivatives Fundamental and applied biological sciences. Psychology Glycocalyx - physiology Polysaccharides - pharmacology Porosity Vertebrates: cardiovascular system |
| title | Permselectivity of Angiogenic Microvessels Following Alteration of the Endothelial Fiber Matrix by Oligosaccharides |
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