Permselectivity of Angiogenic Microvessels Following Alteration of the Endothelial Fiber Matrix by Oligosaccharides

The endothelial glycocalyx, which is composed of integral and peripheral glycoconjugates, forms a fibrous matrix that confers macromolecular sieving properties on the microvascular wall. Changes in pore size within the matrix may regulate macromolecular access to the paracellular and/or vesicular tr...

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Bibliographic Details
Published in:Microvascular research 1997-03, Vol.53 (2), p.150-155
Main Authors: Henry, Charmaine B.S., Durán, Walter N., DeFouw, David O.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood vessels and receptors
Capillary Permeability - physiology
Chick Embryo
Dextrans
Disaccharides - pharmacology
Extravasation of Diagnostic and Therapeutic Materials
Fluorescein-5-isothiocyanate - analogs & derivatives
Fundamental and applied biological sciences. Psychology
Glycocalyx - physiology
Polysaccharides - pharmacology
Porosity
Vertebrates: cardiovascular system
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Summary:The endothelial glycocalyx, which is composed of integral and peripheral glycoconjugates, forms a fibrous matrix that confers macromolecular sieving properties on the microvascular wall. Changes in pore size within the matrix may regulate macromolecular access to the paracellular and/or vesicular transendothelial pathways. We tested the hypothesis that modifications of the endothelial glycocalyx might play a role in the ontogeny of endothelial permselectivity in proliferating microvessels of the chick chorioallantoic membrane (CAM). Accordingly, we evaluated the effects ofDolichos biflorusagglutinin (DBA) orArachis hypogaeaagglutinin (PNA) lectin binding, andN′N′diacetylchitobiose or hydroxyethyl starch polysaccharide (HES) incorporation on CAM endothelial restriction of FITC-dextrans 40 or 150 at Days 4.5 and 5.0 of development. Extravasation of FITC-dextrans was determined by recording their perivascular interstitial intensities. Following DBA, PNA, andN′N′diacetylchitobiose administration, interstitial accumulation of the tracers near first-order pre- and postcapillaries, and surrounding the capillaries, was similar to that of controls at both Days 4.5 and 5.0. At Day 4.5, pretreatment with HES significantly decreased extravasation of FITC-dextran 40. Thus, retention of HES molecules within the glycocalyx might tighten the matrix, and reduce access of dextran 40 to transendothelial pathways across the angiogenic microvessels.
ISSN:0026-2862
1095-9319
DOI:10.1006/mvre.1996.1996