Expression of Krox Proteins During Differentiation of the O‐2A Progenitor Cell Line CG‐4

: Krox proteins are important regulators of development and terminal differentiation. Using the rat glial progenitor cell line CG‐4 as a model system for oligodendrocyte differentiation, we show that on the RNA level Krox‐24 is the predominant member of the Krox family in these cells. Similar result...

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Bibliographic Details
Published in:Journal of neurochemistry 1997-05, Vol.68 (5), p.1911-1919
Main Authors: Sock, Elisabeth, Leger, Hubert, Kuhlbrodt, Kirsten, Schreiber, Jörg, Enderich, Janna, Richter‐Landsberg, Christiane, Wegner, Michael
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Biological and medical sciences
Cell Differentiation
Cell Line
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Early Growth Response Protein 1
Early Growth Response Protein 2
EGR‐1
EGR‐2
Fundamental and applied biological sciences. Psychology
Glia
Immediate-Early Proteins
Isolated neuron and nerve. Neuroglia
Krox‐20
Krox‐24
Mice
Neuroglia - cytology
Neuroglia - metabolism
Oligodendroglia - metabolism
Polymerase Chain Reaction
Rats
Rats, Wistar
RNA, Messenger - metabolism
Stem Cells - cytology
Stem Cells - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Vertebrates: nervous system and sense organs
Zif268
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Summary:: Krox proteins are important regulators of development and terminal differentiation. Using the rat glial progenitor cell line CG‐4 as a model system for oligodendrocyte differentiation, we show that on the RNA level Krox‐24 is the predominant member of the Krox family in these cells. Similar results were also obtained on the protein level as the major Krox protein from CG‐4 cell extracts reacted specifically with an antibody against Krox‐24. Whereas Krox‐24 RNA and protein were abundant in undifferentiated CG‐4 cells, a dramatic decrease in expression was detected after a 3–5‐day period of differentiation during which we observed a reciprocal increase in the levels of myelin basic protein expression. Importantly, regulation of Krox‐24 expression was very similar in CG‐4 cells and primary oligodendrocyte cultures. When expression of Krox‐24 in differentiating CG‐4 cells was followed on a closer time scale, we observed a sharp and transient increase in Krox‐24 RNA, protein, and DNA binding activity immediately after the onset of differentiation followed by an equally rapid decrease. This expression pattern implicates Krox‐24 both in maintenance of the undifferentiated state and in the immediate early phase of differentiation of CG‐4 cells and possibly oligodendrocytes.
ISSN:0022-3042
1471-4159
DOI:10.1046/j.1471-4159.1997.68051911.x