Promoter sequences of the human transforming growth factor-beta 1 gene responsive to transforming growth factor-beta 1 autoinduction

Two distinct regions of the transforming growth factor (TGF)-beta 1 promoter are responsive to autoregulation. Sequences located between nucleotides -454 to -323 and between the two major transcriptional start sites have positive regulatory activities and are induced by TGF-beta 1 in A-549 cells. Th...

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Bibliographic Details
Published in:The Journal of biological chemistry 1989-04, Vol.264 (12), p.7041-7045
Main Authors: Kim, S J, Jeang, K T, Glick, A B, Sporn, M B, Roberts, A B
Format: Article
Language:English
Subjects:
DNA-Binding Proteins - physiology
Gene Expression Regulation
Growth Substances - pharmacology
Humans
Nuclear Proteins - physiology
Promoter Regions, Genetic
Recombinant Fusion Proteins - genetics
Regulatory Sequences, Nucleic Acid
Restriction Mapping
Time Factors
Transcription Factors - physiology
Transcription, Genetic
Transforming Growth Factors - genetics
Tumor Cells, Cultured
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Summary:Two distinct regions of the transforming growth factor (TGF)-beta 1 promoter are responsive to autoregulation. Sequences located between nucleotides -454 to -323 and between the two major transcriptional start sites have positive regulatory activities and are induced by TGF-beta 1 in A-549 cells. The chloramphenicol acetyltransferase activity of the upstream human TGF-beta 1 promoter-chloramphenicol acetyltransferase gene is increased 8- to 10-fold by treatment of cells with TGF-beta 1, whereas that of the second promoter is increased approximately 3- to 4-fold. Using an S1 nuclease protection assay of chloramphenicol acetyl-transferase mRNA, we found that the steady-state expression of chloramphenicol acetyltransferase mRNA also is markedly increased. Seven distinct factors present in nuclear extracts from A-549 cells interact with the sequences between -454 and -323, strongly supporting the involvement of sequence-specific transcription factors in the transcriptional autoactivation of the human TGF-beta 1 gene.
ISSN:0021-9258
1083-351X