Effects of Endurance Training on Mitochondrial Ultrastructure and Fiber Type Distribution in Skeletal Muscle of Patients With Stable Chronic Heart Failure

Objectives. The present study was designed to evaluate the effects of an ambulatory training program in patients with chronic heart failure (CHF) on the ultrastructural morphology of mitochondria and fiber type distribution of skeletal muscle and its relation to peripheral perfusion. Background. Rec...

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Bibliographic Details
Published in:Journal of the American College of Cardiology 1997-04, Vol.29 (5), p.1067-1073
Main Authors: Hambrecht, Rainer, Fiehn, Eduard, Yu, Jiangtao, Niebauer, Josef, Weigl, Claudia, Hilbrich, Lutz, Adams, Volker, Riede, Urs, Schuler, Gerhard
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cardiology. Vascular system
Electron Transport Complex IV - metabolism
Exercise Test
Exercise Therapy
Heart
Heart Failure - pathology
Heart Failure - rehabilitation
Heart failure, cardiogenic pulmonary edema, cardiac enlargement
Humans
Leg - blood supply
Medical sciences
Middle Aged
Mitochondria, Muscle - ultrastructure
Muscle Fibers, Skeletal
Myocardium - enzymology
Oxygen Consumption
Prospective Studies
Pulmonary Gas Exchange
Regional Blood Flow
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Summary:Objectives. The present study was designed to evaluate the effects of an ambulatory training program in patients with chronic heart failure (CHF) on the ultrastructural morphology of mitochondria and fiber type distribution of skeletal muscle and its relation to peripheral perfusion. Background. Recent studies in patients with CHF have suggested that intrinsic abnormalities in skeletal muscle can contribute to the development of early lactic acidosis and fatigue during exercise. Methods. Patients were prospectively randomized to either a training group (n = 9; mean [±SD] left ventricular ejection fraction [LVEF] 26 ± 10%) participating in an ambulatory training program or to a physically inactive control group (n = 9; LVEF 28 ± 10%). At baseline and after 6 months, patients underwent symptom-limited bicycle exercise testing with measurement of central and peripheral hemodynamic variables as well as percutaneous needle biopsies of the vastus lateralis muscle. The mitochondrial ultrastructure of skeletal muscle was analyzed by ultrastructural morphometry; cytochrome coxidase activity was visualized by histochemistry and subsequently quantitated by morphometry. The fiber type distribution was determined by adenosine triphosphatase staining. Results. After 6 months of exercise training there was a significant increase of 41% in the surface density of cytochrome coxidase–positive mitochondria (SVMOcox+) (p < 0.05 vs. control) and of 43% in the surface density of mitochondrial cristae (SVMC) (p < 0.05 vs. control). Furthermore, exercise training induced a 92% increase in the surface density of the mitochondrial inner border membrane (p < 0.05 vs. control). In contrast, the total number of cytochrome coxidase–positive mitochondria remained essentially unchanged. Exercise-induced improvement in peak oxygen uptake was closely linked to changes in SVMOcox+ (p < 0.01, r = 0.66). After exercise training, changes in submaximal femoral venous lactate levels were not related to changes in submaximal leg blood flow (r = −0.4), but were inversely related to changes in the volume density of mitochondria (p = 0.01; r = −0.6) as well as to changes in SVMC (p < 0.05; r = −0.5). After exercise training there was a “reshift” from type II to type I fibers (p < 0.05 vs. control). Conclusions. Patients with CHF who engage in regular physical exercise show enhanced oxidative enzyme activity in the working skeletal muscle and a concomitant reshift to type I fibers. These exercise-ind
ISSN:0735-1097
1558-3597
DOI:10.1016/S0735-1097(97)00015-6