Crystallization and preliminary X-ray analysis of porin from Rhodobacter capsulatus

Porin monomers of the phototrophic bacterium Rhodobacter capsulatus were purified. Crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. The crystals were rhombohedral with an edge length of 0...

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Bibliographic Details
Published in:FEBS letters 1989-01, Vol.242 (2), p.405-408
Main Authors: Nestel, Uwe, Wacker, Thomas, Woitzik, Daniela, Weckesser, Jürgen, Kreutz, Werner, Welte, Wolfram
Format: Article
Language:English
Subjects:
Bacterial Outer Membrane Proteins - ultrastructure
Crystallography
Gram-Negative Bacteria - analysis
Membrane protein crystallization
Molecular Weight
Porin
Porins
Protein Conformation
Rhodobacter capsulatus
X-Ray Diffraction
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Summary:Porin monomers of the phototrophic bacterium Rhodobacter capsulatus were purified. Crystals were obtained from a solution of porin solubilized with the detergent octyltetraoxyethylene within 5 days using the vapor phase equilibration technique. The crystals were rhombohedral with an edge length of 0.4 mm. The space group was trigonal R3 with unit cell constants of a= b=95 Å, c=147 Å. Reflexions were observed to 3.2 Å.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)80511-3