chromosome-specific microdissected library increases marker density on bovine chromosome 1

Genetic resolution of bovine chromosome 1 (BTA1) linkage group was significantly increased by screening for microsatellite clones a microdissected library constructed from a bovine cell line carrying a t(1;29) translocation. Eighty-five percent of the microsatellites (ms) (46/54) identified were inf...

Full description

Saved in:
Bibliographic Details
Published in:Genome research 1997, Vol.7 (1), p.76-80
Main Authors: Sonstegard, T.S, Ponce de León, F. Abel, Beattie, C.W, Kappes, Steven M
Format: Article
Language:English
Subjects:
animal genetics
Animals
Cattle
chromosome mapping
chromosomes
Chromosomes - genetics
DNA libraries
Genetic Markers
Genomic Library
microsatellite repeats
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genetic resolution of bovine chromosome 1 (BTA1) linkage group was significantly increased by screening for microsatellite clones a microdissected library constructed from a bovine cell line carrying a t(1;29) translocation. Eighty-five percent of the microsatellites (ms) (46/54) identified were informative in the USDA/MARC mapping population, and 96% of these ms (44/46) linked to BTA1 (LOD > 3.0). When merged with 40 existing BTA1 markers the genetic map spanned 153.8 cM (sex-averaged interval, 1.9 cM). The fourfold improvement in marker density of BTA1 provides a genetic map that enhances mapping of quantitative trait loci and implementation of marker assisted selection.
ISSN:1549-5469
1088-9051
1549-5469
DOI:10.1101/gr.7.1.76