Comparative sequence analysis and expression of the M6 gene, encoding the outer capsid protein VP5, of African horsesickness virus serotype nine

The entire nucleotide and deduced amino acid sequence of the M6 gene of African horsesickness virus (AHSV) serotype nine has been determined from four overlapping cDNA clones. The gene was found to be 1566 bp long, encoding a protein of 505 amino acids with a molecular weight of 56737 Da and a nett...

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Bibliographic Details
Published in:Virus research 1997, Vol.47 (1), p.41-49
Main Authors: du Plessis, Melinda, Nel, L.H.
Format: Article
Language:English
Subjects:
AFRICAN HORSE SICKNESS VIRUS
African Horse Sickness Virus - classification
African Horse Sickness Virus - genetics
African Horse Sickness Virus - immunology
African horsesickness virus
Amino Acid Sequence
Animals
Antigens, Viral - genetics
Antigens, Viral - immunology
arbovirus
Base Sequence
Capsid - genetics
Capsid - immunology
Capsid Proteins
Cell Line
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
DNA, Viral
EQUIDAE
EXPRESION GENICA
EXPRESSION DES GENES
GENE
GENE EXPRESSION
GENES
Genes, Viral
IMMUNOBLOTTING
MOLECULAR CLONING
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Orbivirus
Outer capsid protein VP5
PROTEINAS
PROTEINE
PROTEINS
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
SECUENCIA NUCLEOTIDICA
Sequence
Sequence Analysis, DNA
Sequence Homology, Amino Acid
SEQUENCE NUCLEOTIDIQUE
Serotyping
Spodoptera - cytology
VIRUS DE LA PESTE EQUINA AFRICANA
VIRUS PESTE EQUINE AFRICAINE
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Summary:The entire nucleotide and deduced amino acid sequence of the M6 gene of African horsesickness virus (AHSV) serotype nine has been determined from four overlapping cDNA clones. The gene was found to be 1566 bp long, encoding a protein of 505 amino acids with a molecular weight of 56737 Da and a nett charge of −1 at neutral pH. Comparative sequence analysis of the deduced amino acid sequence with the VP5 protein of AHSV-4, showed that only 81% of amino acids were conserved in type and position, although alternating regions of lower and higher conservation was identified by alignment of the primary sequences of different orbiviral VP5 proteins. Antigenically authentic AHSV-9 VP5 was also expressed in a baculovirus expression system and the expressed protein was shown to react specifically with anti-AHSV-9 as well as AHSV-3 serum in Western blot analysis.
ISSN:0168-1702
1872-7492
DOI:10.1016/S0168-1702(96)01400-1